The normal uroepithelial cell SV-HUC-1 was purchased from the Cell Bank of the Chinese Academy of Sciences, and bladder cancer cell lines UM-UC-3, 253 J, T24, and J82 were purchased from ATCC (Rockville, Maryland). The 293 A cell line was collected in our lab. All cells were cultured in DMEM supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. Cells were grown in a humidified atmosphere of 95% air and 5% CO2. Transfection was performed by using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocols. The miR-625-5p, miR-149-3p, and miR-449a mimic, mimic NC, miR-625-5p inhibitor, inhibitor NC, shRBM24, shRunx1t1, shTCF4, and negative controls were purchased from GenePharma Co., Ltd. (Shanghai, China). The oeRBM24 and oeRunx1t1 overexpression plasmids were obtained from GENEWIZ Company (Suzhou, China).
Shrbm24
Manufactured by GenePharma
Sourced in China
The ShRBM24 is a laboratory equipment product designed for specialized research applications. It functions as a high-performance, automated RNA interference (RNAi) screening platform. The core purpose of this device is to facilitate efficient and precise gene silencing experiments in a variety of cell lines and model systems.
Automatically generated - may contain errors
Lab products found in correlation
Umuc3, by American Type Culture Collection (2 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions)
Sv huc 1, by National Collection of Authenticated Cell Cultures (2 mentions)
Shrunx1t1, by GenePharma (2 mentions)
Shtcf4, by GenePharma (2 mentions)
Oerbm24, by Genewiz (2 mentions)
Inhibitor nc, by GenePharma (1 mentions)
Mimic nc, by GenePharma (1 mentions)
2 protocols using shrbm24
1
Bladder Cancer Cell Line Transfection Protocols
2
Bladder Cancer Cell Line Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Normal uroepithelial cell SV-HUC-1 was purchased from the Cell Bank of the Chinese Academy of Sciences and bladder cancer cell lines UM-UC-3, 253J,T24 and J82 were purchased from ATCC (Rockvill, Maryland) and 293A cell line collected in our lab, and all cells cultured in DMEM supplemented with 10% Fetal Bovine Serum (FBS), 1% penicillin/streptomycin. Cells were grown in a humidi ed atmosphere of 95% air and 5% CO2. The transfection was performed according to the manufacturer's protocols by using Lipofectamine 2000 (Invitrogen). The miR-625-5p, miR-149-3p and miR-449a mimics, mimic NC, miR-625-5p inhibitors, inhibitor NC, shRBM24, shRunx1t1, shTCF4 and negative controls were purchased from GenePharma Co., Ltd (Shanghai, China). The over-expression plasmids of oeRBM24, oeRunx1t1 was obtained from GENEWIZ Company (Suzhou, China) .
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