Poly (lactic-co-glycolic acid) (PLGA) (50:50, Mw = 12,000) was purchased from Boehringer Ingelheim (Ingelheim, Germany). Ranibizumab was a generous gift from Genentech (San Francisco, CA). Dichloromethane (DCM), acetonitrile, ethylene glycol (EG), and Dimethyl sulfoxide (DMSO) were purchased from Fisher Scientific (Newton, NJ). The fluorescence dye 1,1'-Dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (‘DiI’, D-282) was purchased from Invitrogen (Grand Island, NY) and Nile red from Sigma-Aldrich (St. Louis, MO). Rhodamine-6G was purchased from Acros organics (Fisher Scientific Newton, NJ). The Enzyme-linked immunosorbent assay (Elisa) plate was purchased from Greiner Bio-one (Monroe, NC).
Poly lactic co glycolic acid plga
Manufactured by Boehringer Ingelheim
Sourced in Germany, United States
Poly (lactic-co-glycolic acid) (PLGA) is a biodegradable and biocompatible copolymer. It is composed of lactic acid and glycolic acid monomers. PLGA is a widely used material in various biomedical applications, including drug delivery systems, tissue engineering, and medical devices.
Automatically generated - may contain errors
Lab products found in correlation
Milli q, by Merck Group (2 mentions)
Nile red, by Merck Group (1 mentions)
Elisa plate, by Greiner (1 mentions)
Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (1 mentions)
Acetonitrile, by Thermo Fisher Scientific (1 mentions)
Ethylene glycol, by Thermo Fisher Scientific (1 mentions)
Dichloromethane, by Thermo Fisher Scientific (1 mentions)
Ranibizumab, by Genentech (1 mentions)
Polyvinyl alcohol (pva), by Merck Group (1 mentions)
Caym 14010, by Cayman Chemical (1 mentions)
Polycaprolactone, by Merck Group (1 mentions)
Tgf β3, by Thermo Fisher Scientific (1 mentions)
Poly methyl vinyl ether alt maleic anhydride, by Merck Group (1 mentions)
3 protocols using poly lactic co glycolic acid plga
1
PLGA-Based Drug Delivery System
2
Preparation of LTB4-loaded PLGA Microspheres
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Microspheres (MS) were prepared as a pharmacological strategy using an oil-in-water emulsion solvent extraction-evaporation process (Nicolete et al., 2007; Nicolete et al., 2008) . Briefly, LTB 4 was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
The copyright holder for this preprint (which this version posted May 24, 2020. ; https://doi.org/10.1101/2020.05.22.111633 doi: bioRxiv preprint 5 (CAYM-14010; Cayman Chemical Company, Michigan, USA) was dissolved in absolute ethanol (100 µg/mL). Then, 0.3 mL of the organic phase, equivalent to 3× 10 -5 M of the LTB 4 solution was added to 10 mL of methylene chloride supplemented with 30 mg of 50:50 poly (lactic-co-glycolic acid) (PLGA) (Boehringer Ingelheim, Germany). Next, 40 mL of 3% polyvinyl alcohol (3% w/v PVA) (Sigma-Aldrich CO., St. Louis, MO, USA) were added and the mixture was mechanically stirred at 600 rpm for 4 h (RW-20; Ika®-Werke GmbH & CO. KG, Staufen, Germany).
Microspheres were washed (3x) with deionized water (Milli-Q®, Merck Millipore, Darmstadt, Germany), lyophilized, and stored at -20 °C until use.
The copyright holder for this preprint (which this version posted May 24, 2020. ; https://doi.org/10.1101/2020.05.22.111633 doi: bioRxiv preprint 5 (CAYM-14010; Cayman Chemical Company, Michigan, USA) was dissolved in absolute ethanol (100 µg/mL). Then, 0.3 mL of the organic phase, equivalent to 3× 10 -5 M of the LTB 4 solution was added to 10 mL of methylene chloride supplemented with 30 mg of 50:50 poly (lactic-co-glycolic acid) (PLGA) (Boehringer Ingelheim, Germany). Next, 40 mL of 3% polyvinyl alcohol (3% w/v PVA) (Sigma-Aldrich CO., St. Louis, MO, USA) were added and the mixture was mechanically stirred at 600 rpm for 4 h (RW-20; Ika®-Werke GmbH & CO. KG, Staufen, Germany).
Microspheres were washed (3x) with deionized water (Milli-Q®, Merck Millipore, Darmstadt, Germany), lyophilized, and stored at -20 °C until use.
3
Biomaterial-Based Growth Factor Delivery
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Soy lecithin, heparin sodium porcine mucosa, ethylenediamine tetrakis(propoxylateblock-ethoxylate) tetrol (Tetronic 1107 ® , T1107), polyoxyethylene (20) (link) sorbitan monolaureate (Tween ® 20), cottonseed oil, polycaprolactone (PCL, Mn 70000 -90000 Da), poly(methyl vinyl ether-alt-maleic) anhydride (PVM/MA, Mn 80000 Da), methoxypolyethylene glycol amine 5000 (PEGAm), dextran sulfate sodium salt (Mw > 500000) were all purchased from Sigma-Aldrich (Missouri, USA). Poly(lactic-coglycolic acid) (PLGA) was obtained from Boehringer-Ingelheim (Petersburg, USA).
Recombinant proteins BMP-7 and TGF-β3 were bought from Peprotech (USA); their respective ELISA kits were bought from Abnova (Taiwan) and Uscn Life Sience Inc.
(China). The remaining reagents and solvents used were of analytical grade or better. In all the experiments, ultrapure water was employed (Milli-Q, Millipore).
Recombinant proteins BMP-7 and TGF-β3 were bought from Peprotech (USA); their respective ELISA kits were bought from Abnova (Taiwan) and Uscn Life Sience Inc.
(China). The remaining reagents and solvents used were of analytical grade or better. In all the experiments, ultrapure water was employed (Milli-Q, Millipore).
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