The human GC cell lines HGC-27, SGC-7901, MGC-803, MKN-45, and NCI-N87 were purchased from the American Type Culture Collection (Manassas, United States). HGC-27, NCI-N87, and MGC-803 cells were cultured in Dulbecco's modified Eagle's medium containing 10% fetal bovine serum (FBS); Biological Industries, Israel). SGC-7901 and MKN-45 cells were grown in RPMI 1640 medium containing 10% FBS. Trastuzumab-resistant HGC-27 and SGC-7901 cells were established by culturing cells with increasing concentrations of Trastuzumab (Roche, Switzerland) over half a year and were designated HGC-27-R and SGC-7901-R cells, respectively. All cells were cultured in incubators at 37 ℃ with 5% CO2. Thioridazine and lapatinib were obtained from Selleck Chemicals (United States).
Yang Z.Y., Zhao Y.W., Xue J.R., Guo R., Zhao Z., Liu H.D., Ren Z.G, & Shi M. (2023). Thioridazine reverses trastuzumab resistance in gastric cancer by inhibiting S-phase kinase associated protein 2-mediated aerobic glycolysis. World Journal of Gastroenterology, 29(45), 5974-5987.
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