Atr ftir vertex 70 5 spectrometer

Manufactured by Bruker

The ATR-FTIR Vertex 70 V spectrometer is a Fourier-transform infrared (FTIR) spectrometer designed for attenuated total reflection (ATR) analysis. It is capable of collecting infrared absorption spectra of solid, liquid, and gas samples.

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Lab products found in correlation

Opus software, by Bruker (1 mentions) Matlab 2016a, by MathWorks (1 mentions)

Close spelling variants of this product

Vertex 70 (1011 citations) Vertex 70 FTIR spectrometer (297 citations) Vertex 70 spectrometer (284 citations) FTIR spectrometer (163 citations) Bruker spectrometers (71 citations) Vertex 70 FTIR (58 citations) ATR-FTIR (31 citations) ATR-FTIR spectrometer (13 citations) Vertex 70 ATR-FTIR (4 citations) VERTEX 70 ATR–FTIR spectrometer (2 citations)

2 protocols using atr ftir vertex 70 5 spectrometer

Hydration-Dependent ATR-FTIR Analysis of Biopolymers

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To dynamically determine the hydrating states of biopolymers, before ATR-FTIR analysis, the following quantities of double-distilled water were added to the lyophilized BS-SBP3 or EPS-SBP3: 0, 20, 40, 60, 80, 100, 120, 140, and 160% w/w [52 (link)].
ATR-FTIR spectroscopy allows obtaining qualitative information about the anhydrous BS-SBP3 or EPS-SBP3 and the two hydrated biopolymers. To collect the spectra of anhydrous and progressively hydrated BS-SBP3 or EPS-SBP3, an ATR-FTIR Vertex 70 V spectrometer from Bruker Optics with a platinum diamond was used. The ATR-FTIR spectra were collected using an average of 64 scans with a resolution of 4 cm⁻¹ in the spectral range of 500 ÷ 4000 cm⁻¹. To decrease the instrumental noise of the spectra, the data were pre-processed using Bruker OPUS software, performing (i) the baseline and (ii) the smoothing treatments. Finally, a procedure to normalize the spectra, with specific reference to some bands having the same intensity in each region, has allowed reducing the differences between one measurement and another [53 (link)]. After this procedure, data were processed in a MATLAB environment.

Caccamo M.T., Zammuto V., Spanò A., Gugliandolo C, & Magazù S. (2022). Hydrating Capabilities of the Biopolymers Produced by the Marine Thermophilic Bacillus horneckiae SBP3 as Evaluated by ATR-FTIR Spectroscopy. Materials, 15(17), 5988.

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ATR-FTIR Analysis of Carbohydrate Interactions

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ATR-FTIRVertex 70V spectrometer (Bruker Optics) using Platinum diamond ATR was employed to collect spectra on EPS1-T14, fructose, fucose and glucose, in the temperatures range from 20° C to 80 °C. All the spectra were obtained by collecting 48 scans with a resolution of 4 cm -1 in the 4000 to 400 cm -1 wavenumber range. The following data pre-processing procedures were applied: i) a baseline correction, in order to diminish the dissimilarities between spectra due to baseline shift; ii) a smoothing treatment, to reduce the instrumental noise; iii) a first derivative treatment, to correct baseline shift together, and a second derivative treatment, to better discriminate features associated with spectra; iv) a spectral normalization, to correct the path-length variation and to reduce the differences among single measurements [27] [28] [29] .
The intramolecular OH-stretching band frequency shift evaluation and the wavelet cross-correlation analysis were performed by MATLAB 2016a software (Mathworks, Natick, USA). The spectral distance calculation was performed by Origin 9 software package (OriginLab Co., Northampton, USA).

Caccamo M.T., Zammuto V., Gugliandolo C., Madeleine-Perdrillat C., Spanò A, & Magazù S. (2018). Thermal restraint of a bacterial exopolysaccharide of shallow vent origin. International journal of biological macromolecules, 114.

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