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2 protocols using conc sulphuric acid

1

Antibody-Antigen Assay with MWCNT

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Alpha-fetoprotein antibody and antigen were purchased from Biocell Co. (Zhengzhou, China). Bovine serum albumin (BSA, 96-99%) and multi-walled carbon nanotubes (MWCNT), carbon > 95% 6 -9 nm O.D x 5 µm L were procured from Sigma (USA).
Phosphate buffer solution (PBS), conc. sulphuric acid (H 2 SO 4 ), 88% ACS ortho phosphoric acid (H 3 PO 4 ), iron sulphate (FeSO 4 ), potassium permanganate (KMnO 4 ), 30% hydrogen peroxide (H 2 O 2 ), 35% hydrochloric acid (HCl), 98% hydrazine hydrate (NH 2 ) 2 : H 2 O, chloroauric acid (HAuCl 4 ) potassium ferrocyanide (K 4 Fe(CN) 6 ) and potassium ferricyanide (K 3 Fe(CN) 6 ) were purchased from Merck India. All the electrolytes were prepared with ultrapure water (>18 M Ω cm) from a Millipore Milli-Q water purification system.
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2

Extraction and Analysis of Organic Compounds

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Petroleum ether (60°-80°C), methanol (MeOH), potassium hydroxide (KOH), conc. sulphuric acid (H 2 SO 4 ), anhydrous sodium sulphate (Na 2 SO 4 ), acetic anhydride, n-hexane, diethyl ether, glacial acetic acid, chloroform, and benzene were purchased from Merck (India).
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