Mini protean precast tgx gels 8 16

Cellulose nanocrystal powder (CNCs) was purchased from CelluForce™, Canada According to the manufacturer; it contained of 100% sulphated CNCs. Whey protein isolate (WPI) powder containing 96.3 wt% protein was kindly gifted by Fonterra Limited (Auckland, New Zealand). Microcrystalline cellulose (MCC), produced by acid hydrolysis of cellulose, product code 310697 and pure -lactoglobulin ( -lg), ≥ 90% (PAGE), product code L3908, were purchased from Sigma Aldrich, (New Jersey USA). Sunflower oil was purchased from a local supermarket (Morrisons, UK).
Pepsin enzyme (P7000-25G, actual activity: 474 U mg -1 ) was purchased from Sigma-Aldrich Company Ltd, Dorset, UK. All other chemicals used were of analytical grade unless otherwise specified. Mini-Protean Precast TGX Gels (8-16%) and Precision Plus Protein All Blue Standards were purchased from Bio-Rad Laboratories, Inc, USA. Milli-Q water having an ionic purity of 18.2 M cm at 25 °C (water purified by treatment with a Milli-Q apparatus) was used for all the experiments.

Mini-Protean Precast TGX gels (8-16%) and Precision Plus Protein All Blue Standards were purchased from Bio-Rad (Bio-Rad Laboratories Ltd., Richmond, CA, USA). All reagents were of analytical grade and used without further purification unless otherwise reported. All solutions were prepared with Milli-Q water with a resistivity of 18.2 M cm at 25 °C (Milli-Q apparatus, Millipore, Bedford, UK). Sodium azide (0.02 wt %) was added as a preservative.