Cellulose adsorbent pad

Cortisol, polyclonal anti-HRP antibody produced in rabbit, bovine serum albumin (BSA), and Tween-20 were purchased from Sigma Aldrich (St. Louis, MO). Western Lightning Ultra CL substrate for HRP was purchased from Perkin Elmer (Waltham, MA). Polyclonal antiCortisol antibody produced in rabbit was purchased from Analytical Antibodies (Bologna, Italy). HRP-conjugate Cortisol was generously provided by Diametra (Milano, Italy). Phosphate buffered saline (PBS) was prepared as follows: 10 mmol L -1 Na2HPO4, 2 mmol L -1 KH2PO4, 137 mmol L -1 NaCl, 2.7 mmol L -1 KCl, pH 7.4. The other reagents were of analytical grade and were employed as received.
Materials for the LFIA strip: glass fiber pad, cellulose pad, nitrocellulose membrane (Hi-flow plus 180 membrane cards), and cellulose adsorbent pad were from Merck Millipore (Billerica, MA); saliva sample pad I (Whatman LF1) and saliva sample pad II (Whatman Standard 14) were provided by GE Healthcare Life Science (UK).

Fumonisin B1, zearalenone, aflatoxin B1, deoxynivalenol (Oekanal standard solutions), polyethylene glycol (PEG, average mw 10 kDa), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC), cadmium oxide (CdO, 99,99%), selenium powder (Se, 99,99%), sulfur powder (S, 99%), zinc acetate (Zn(OAc) 2 , 99,99%), oleic acid (90%), 1-octadecene (90%), oleylamine (70%), octadecylamine (90%), and trioctylphosphine, poly(maleic anhydride-alt-1-octadecene) (PMAO, M ~ 30 000-50 000) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Tween 20, Triton X-100 and other chemicals were purchased from Merck-VWR (Milan, Italy).
Polyclonal antibodies towards Fumonisin B1 Ab vs FM developed in rabbit and BSA-FMB1 were kindly provided by Generon srl (Modena, Italy). The -globulin fraction was isolated by ammonium sulfate precipitation and used without any additional treatments. Jeffamine M1000 (1000 g/mol) was kindly provided by Huntsman (Belgium).
Millipore High Flow (HF) 240, 180, 120 and 90, and cellulose adsorbent pad were obtained from Merck Millipore (Billerica, MA, USA).

The polyclonal anti-OTA antibody produced in rabbit and the HRP-OTA conjugate were kindly provided by Euroclone (Milan, Italy). Polyclonal anti-HRP antibody produced in rabbit, ovalbumin (OVA), Tween-20, and polyethylene glycol 10000 were obtained from Sigma-Aldrich Co (St. Louis, MO). The CL HRP detection substrate Supersignal ELISA Femto was purchased from Thermo Scientific Inc. (Rockford, IL). The other reagents were of analytical grade and were employed as received. Phosphate buffered saline (PBS) contained 10 mmol L -1 Na2HPO4, 2 mmol L -1 KH2PO4, 137 mmol L -1 NaCl, 2.7 mmol L -1 KCl, with pH adjusted to 7.4. The LFIA strips were produced using Whatman Standard 14 glass fibre sample pad (GE Healthcare Lifescience, Chalfont St. Giles, UK), Hi-flow plus 180 nitrocellulose membrane cards (Merck Millipore, Billerica, MA), and cellulose adsorbent pads (Merck Millipore).