Cells were doubly incubated with FITC-conjugated anti-CD45R (1 mg/10 6 cells; eBioscience, Thermo Fisher Scientific, Waltham, MA) and PE-conjugated anti-IgM (1 mg/10 6 cells; BD Biosciences, Heidelberg, Germany), FITC-conjugated anti-CD45R (1 mg/10 6 cells; eBioscience) and PE-conjugated anti-CD138 (1 mg/10 6 cells; Abcam, Cambridge, UK), FITC-conjugated anti-CD3 (1 mg/10 6 cells; eBioscience) and PE-conjugated anti-CD4 (0.5 mg/10 6 cells; eBioscience) as well as FITC-conjugated anti-CD3 (1 mg/10 6 cells; eBioscience) and PE-conjugated anti-CD8 (0.125 mg/10 6 cells; eBioscience), respectively. The percentages of
T cells were determined through a lymphocyte gate by flow cytometry (BD Accuri C6, BD Biosciences).
Li J., Basler M., Alvarez G., Brunner T., Kirk C.J, & Groettrup M. (2018). Immunoproteasome inhibition prevents chronic antibody-mediated allograft rejection in renal transplantation. Kidney international, 93(3).
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