A Multimode ™ AFM with a Nanoscope IIIa controller (Veeco, Santa Barbara, CA) operated in contact mode was used to image pseudoviruses before and after addition of vimentin or DNA. The cantilevers used were silicon nitride with a spring constant of 0.35 N/m (Veeco, Santa Barbara, CA). The AFM was calibrated using a 3D reference of 200 nm height and 10 µm pitch (Digital Instruments, Santa Barbara, CA). Deflection and height images were obtained with scan rates of 1 Hz with a resolution of 512 pixels/line. Briefly, for AFM evaluation of pseudoviruses in control (non-vimentin addition) and samples preincubated with human recombinant vimentin, a 10 µl drop of pseudoviruses or pseudoviruses/vimentin was applied on cleaved mica (SPI Supplies, West Chester, PA) and analyzed in a dried environment.
Cleaved mica
Manufactured by SPI Supplies
Sourced in Cameroon
Cleaved mica is a naturally occurring mineral that can be easily split into thin, transparent sheets. It is a common material used in various laboratory applications due to its unique physical properties.
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Lab products found in correlation
3 protocols using cleaved mica
1
Atomic Force Microscopy of Pseudoviruses
2
Topographic Imaging of Oxidized Chlorophyll Complexes
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A 100 μL aliquot of oxidized molischianum-LH2 solution in 20 mM Tris buffer containing 0.02% DDM and 150 mM NaCl (pH 8.0) was placed onto a cleaved mica (SPI Supplies). After standing for 30 min at room temperature in the dark, the mica surface was rigorously rinsed with 20 mM Tris buffer containing 150 mM NaCl (pH 8.0). The oxidized molischianum-LH2 was then observed in 20 mM Tris buffer containing 150 mM NaCl (pH 8.0) by a laboratory-built frequency modulation AFM controlled by a commercially available AFM controller (ARC2, Asylum Research)29 (link) with a silicon cantilever with gold coating of deflection side (160 AC-NG, MikroMasch), which had a nominal spring constant of 26 N/m. The typical resonance frequency and Q factor in an aqueous buffer solution are 120 kHz and 7, respectively.
3
Multimode AFM Imaging of Pseudoviruses
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A Multimode™ AFM with a Nanoscope Ilia controller (Veeco, Santa Barbara, CA) operated in contact mode was used to image pseudoviruses before and after addition of vimentin or DNA. The cantilevers used were silicon nitride with a spring constant of 0.35 N/m (Veeco, Santa Barbara, CA). The AFM was calibrated using a 3D reference of 200 nm height and 10 μm pitch (Digital Instruments, Santa Barbara, CA). Deflection and height images were obtained with scan rates of 1 Hz with a resolution of 512 pixels/line. Briefly, for AFM evaluation of pseudoviruses in control (non-vimentin addition) and samples preincubated with human recombinant vimentin, a 10 μl drop of pseudoviruses or pseudoviruses/vimentin was applied on cleaved mica (SPI Supplies, West Chester, PA) and analyzed in a dried environment.
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