BT474 human breast cancer cells (HER2-overexpression) were obtained from the American Type Culture Collection (catalog number HTB-20, ATCC) and were cultured in RPMI 1640 supplemented with 10% FBS. Wild-type (WT) SKBR3 human breast cancer cells (catalog number HTB-30, ATCC) were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS). Trastuzumab-resistant (TR) SKBR3 cells were established by continuous culture of WT SKBR3 cells in the presence of 5 μg/ml trastuzumab for 6 months in a humidified atmosphere of 5% CO2 and 95% air at 37 °C according to our previous reports1 (link),3 (link).
Skbr3 human breast cancer cells
Manufactured by American Type Culture Collection
Sourced in United States
SKBR3 human breast cancer cells are a well-established cell line derived from a human breast adenocarcinoma. They are widely used in cancer research as a model for studying breast cancer biology and developing therapeutic interventions.
Automatically generated - may contain errors
Lab products found in correlation
Bt474 human breast cancer cells, by American Type Culture Collection (1 mentions)
Penicillin streptomycin, by Corning (1 mentions)
293t cell, by American Type Culture Collection (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Dulbecco s minimal essential medium, by Merck Group (1 mentions)
Mda mb 468, by American Type Culture Collection (1 mentions)
Mcf 7, by American Type Culture Collection (1 mentions)
Paclitaxel, by Merck Group (1 mentions)
Dulbecco s modified eagle medium, by Cytiva (1 mentions)
5 protocols using skbr3 human breast cancer cells
1
Establishing Trastuzumab-Resistant Breast Cancer Cells
2
Culturing SKBR3 Human Breast Cancer Cells
3
Culturing SKBR3 and 293T Cell Lines
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SKBR3 human breast cancer cells (American Type Culture Collection (ATCC), Rockville, MD) were grown in McCoy's 5A media supplemented with 10% fetal bovine serum (FBS) and L-glutamate. 293T cells (American Type Culture Collection (ATCC), Rockville, MD) were grown in DMEM with 10% FBS and L-glutamate.
4
E. coli Culture and Breast Cancer Cell Growth
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E. coli BL21 [F-ompT hsdSB (rB−, mB−) gal dcm (DE3), Novagen, San Diego, USA] was used in this study. MDA-MB-468 and SK-BR-3 human breast cancer cells (American Type Culture Collection, Manassas, VA, USA) were cultured in Dulbecco’s minimal essential medium (Sigma, St Louis, MO, USA) supplemented with 10% heat-inactivated bovine serum, 100 units/mL penicillin, and 100 µg/mL streptomycin (Gibco Laboratories, Grand Island, NY, USA) at 37°C in a humidified 5% CO2 atmosphere.
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E. coli BL21 [F-ompT hsdSB (rB−, mB−) gal dcm (DE3), Novagen, San Diego, USA] was used in this study. MDA-MB-468 and SK-BR-3 human breast cancer cells (American Type Culture Collection, Manassas, VA, USA) were cultured in Dulbecco’s minimal essential medium (Sigma, St Louis, MO, USA) supplemented with 10% heat-inactivated bovine serum, 100 units/mL penicillin, and 100 µg/mL streptomycin (Gibco Laboratories, Grand Island, NY, USA) at 37°C in a humidified 5% CO2 atmosphere.
5
Establishing Paclitaxel-Resistant Breast Cancer Cell Lines
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MCF-7 and SKBR3 human breast cancer cells were obtained from the American Type Culture Collection (Manassas, VA, USA). Cells were cultured in Dulbecco's modified Eagle medium (containing 25 mM glucose and 10% fetal bovine serum) (GE Healthcare Life Sciences HyClone Laboratories, Logan, UT, USA) at 37°C and 5% CO2. To establish PR cell lines, the cells were incubated with 1 µM paclitaxel (Sigma-Aldrich; Merck Millipore, Darmstadt, Germany) for different days. The culture medium was changed every 2 days in the presence of 1 µM paclitaxel.
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