Bx51 biomicroscope

Ten hours before plucking, three rabbits were treated with oral dexamethasone 1.5 mg and about 30 cm² hair was plucked to initiate a new hair cycle. Oral dexamethasone dilated hair follicles and made hair easily to be pulled out. Meanwhile, the manipulation should be done correctly and the strength should be moderate when pluck hairs. Hold the rabbit in place with your left hand, pinch the hair fibers with your index, middle and thumb fingers of right hand, and pull it out a pinch by a pinch. Rabbits were given anaesthesia through an ear vein injection of 0.7% pentobarbital sodium (6 ml/kg) before sampling. Skin tissue samples (about 1 cm²) from the back of each rabbit were collected in the first, fourth, eighth, and twenty-fourth weeks after plucking hairs. The skin samples were fixed in 4% paraformaldehyde solution for histological analysis. Cross sections of the fixed samples were washed with running water, dehydrated using an ethyl alcohol series, cleaned in xylene, and embedded in paraffin wax. The specimens were sectioned to a thickness of 4 μm using a Leica RM2235 microtome (Leica, Wetzlar, Germany). Transverse and vertical cross-sections of the fixed and paraffin-embedded samples were stained with haematoxylin-eosin (HE), examined, and photographed using an Olympus BX51 biomicroscope (Olympus Optical Company, Tokyo, Japan).

Rabbits were given anesthesia through an ear vein injection of 0.7% pentobarbital sodium (6 ml/kg) before sampling. Skin tissue samples (1 cm²) from the back of each rabbit were collected at week four, six and eight after plucking hairs for histological analysis. The skin samples were fixed with 4% formaldehyde (40% formaldehyde solution and distilled water mixed at a 1:9 ratio) solution. Cross sections of the fixed samples were washed with running water, dehydrated using an ethyl alcohol series, cleaned in xylene, and embedded in paraffin wax. The specimens were sectioned to a thickness of 4 μm using a Leica RM2235 microtome (Leica, Wetzlar, Germany). Transverse and vertical cross-sections of the fixed and paraffin-embedded samples were stained with HE, examined and photographed using an Olympus BX51 biomicroscope (Olympus Optical Company, Tokyo, Japan).