Trizma Base, EDTA, sodium hydroxide, sodium dodecyl sulphate, glacial acetic acid, ethidium bromide, imidazole, sodium chloride, potassium chloride, glycerol, acrylamide, bis-acrylamide, ammonium persulphate, TEMED, glycine, bovine serum albumin, β-mercaptoethanol, hydrogen peroxide (H
2O
2), 8-anilino-1-naphthalenesulfonic acid ammonium salt (ANS), reduced glutathione (GSH), glutathione reductase, reduced β-nicotinamide adenine dinucleotide phosphate (β-NADPH), dithiothreitol (DTT), guanidinium chloride (GdmCl), luria bertani medium and luria bertani agar (BD Difco, USA), PBS tablets (Bio Basic),
Ni-NTA resin (Qiagen GmbH, Germany),
DNA markers (GeneDireX Inc., USA),
precision dual colour protein marker (Bio-Rad), ampicillin and kanamycin (MP Biomedicals), HiYield PlusTM plasmid mini isolation kit (RBC Bioscience), bradford protein estimation kit (MP Biomedicals),
EnzCheck phospholipase A2 assay kit (Invitrogen). Primary (monoclonal anti- hPrdx6, clone 3A10-2A11 antibody produced in mouse, WH0009588M1) and secondary (anti-mouse IgG (Fab Specific)-peroxidase antibody produced in goat, A3682) antibody procured from Sigma-Aldrich,
E.coli strains: DH5α and M15[pREP4].
Chowhan R.K., Hotumalani S., Rahaman H, & Singh L.R. (2021). pH induced conformational alteration in human peroxiredoxin 6 might be responsible for its resistance against lysosomal pH or high temperature. Scientific Reports, 11, 9657.