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2 protocols using pseudomonas fluorescens

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Antimicrobial Activity Evaluation

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The chemicals that are used in the present study were purchased from Himedia Laboratories Pvt. Ltd., Mumbai, India. The pathogens Bacillus subtilis (MTCC 10403), Escherichia coli (MTCC 443), Pseudomonas aeruginosa (MTCC 424), Micrococcus luteus (MTCC 1809), Staphylococcus aureus (MTCC 1144), Salmonella enterica typhimurium (MTCC 98), Klebsiella pneumonia (MTCC 10309), Bacillus cereus (MTCC 1272), Klebsiella aerogenes (MTCC 2822), and Pseudomonas fluorescens (MTCC 667) were received from Microbial Type Culture Collection (MTCC) and Gene Bank, Chandigarh, India. In the current study, the instruments used were microplate reader (Multiskan FC, Thermo Fisher Scientific, Mumbai, India), centrifuge (Remi CPR-30 plus, Mumbai, India), Vortex Shaker (SPINIX, Kolkata, India), PVDF syringe filter (0.22 μm; Thermo Fisher Scientific, Mumbai, India), CO2 incubator (Thermo Fisher Scientific, Mumbai, India), and shaking incubator (Thermo Fisher Scientific, Mumbai, India).
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2

Bacterial Cultures for Antimicrobial Assays

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Staphylococcus epidermidis (MTCC No. 435), Escherichia coli (MTCC No. 443), Micrococcus luteus (MTCC No. 106) and Pseudomonas fluorescens (MTCC No. 2421) from Microbial Type Culture Collection (MTCC), Chandigarh were used in the current study. Bacteria were grown in nutrient broth at 37 °C for 16-18 hours. ML-35p E. coli strain was kindly gifted by Dr. Dinkar Sahal from The International Centre for Genetic Engineering and Biotechnology, New Delhi, India.
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