Oxybuprocaine hydrochloride solution

We used a modified Schirmer test on rat eyes to measurement tear fluid secretion under topical anesthesia by 0.4% oxybuprocaine hydrochloride solution (Santen Pharmaceutical, Japan) [24] (link). A phenol red thread (Zone-Quick; Showa Yakuhin kako, Japan) was placed on the temporal side of the upper eyelid margin for 1 minute. The length of the moistened area from the edge was measured within 1 mm.

In the mouse study, tear secretion was measured using a phenol red thread (Zone-Quick; Showa Yakuhin Kako Co., Ltd., Tokyo, Japan). The threads were placed on the medial canthus for 15 s. The length of the moistened area from the edge was then measured to within 0.5 mm. Tear secretion was measured before the oil product administration and after the end of stress exposure. The data are represented as percentages of the pre-administration values. For the mouse tear secretion measurement, five to seven mice were used from each group.
In the rat study, modified Schirmer test was used under topical anesthesia induced with a 0.4% oxybuprocaine hydrochloride solution (Santen Pharmaceutical, Osaka, Japan). Tear secretion was measured before the oil product administration of every treatment day and 18 h after the last time the rat was kept stationary on a swing on day 10 (day 11). After 3 min of anesthesia, a phenol red thread (Zone-Quick; Syowa Yakuhin Kako, Ltd., Tokyo, Japan) was placed on the temporal side of the lower eyelid margin for one minute. The length of the moistened area from the edge was then measured to within 1 mm. For the rat tear secretion measurement, six rats were used from each group.