Slides were fixed in 4% paraformaldehyde in 13 PBS, blocked in Superblock (Thermo Scientific), and then incubated overnight at 4°C in 1:200 monoclonal mouse immunoglobulin G (IgG) 2b anti-human-HuC/D (Invitrogen) or 1:500 polyclonal rabbit IgG anti-GFP (Molecular Probes) in PBS (5% horse serum, 0.16% Triton X-100 in 1X PBS). Slides were then incubated in 1:500 goat anti-mouse Alexa 568 IgG (Molecular Probes) or 1:500 donkey anti-rabbit Alexa 488 IgG (Molecular Probes), DAPI stained, and coverslipped with Gelvatol.
Igg 2b anti human huc d
Manufactured by Thermo Fisher Scientific
The IgG2b anti-human-HuC/D is a primary antibody that specifically binds to the HuC/D proteins, which are markers for neuronal cell bodies and dendrites. This antibody is commonly used in immunohistochemical and immunofluorescence applications for the identification and localization of neurons in tissue samples.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using igg 2b anti human huc d
1
Immunofluorescent Labeling of Cultured Cells
2
Immunofluorescent Labeling of Cultured Cells
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