Mir 200c 3p

n-Cetyltrimethylammonium
bromide (CTABr; purity ≥98%), tetraethyl orthosilicate (TEOS;
purity: 98%), sodium hydroxide (NaOH; purity ≥98%), polyethylenimine
(PEI; average molecular weight: 25 000 g/mol), hyaluronic acid
(HA; #53747), propidium iodide (PI; purity ≥94%), diamidino-2-phenylindole
dihydrochloride (DAPI; purity ≥98%), lysosome Isolation Kit
(LYSISO1), and tetramethylammonium hydroxide solution (TMAH: 25 wt
% in H₂O) were purchased from Sigma-Aldrich (St. Louis,
MO). Tetramethylrhodamine-5-isothiocyanate (TRITC; T490), RNA labeled
with Cy3 (miRCy3, AM4621), DMEM/F12, RPMI 1640, l-glutamine,
penicillin-streptomycin (10000 U/mL), fetal bovine serum (FBS), formaldehyde
(28908), LysoTracker Deep Red (L12492), Hoechst33342, scramble miRNA
(4464058), and Lipofectamine 2000 were acquired from Thermo Fisher
Scientific (Waltham, MA). miR-200c-3p was acquired from Guangzhou
RiboBio CO (Guangzhou, China).

Wild-type C57BL/6 mice were purchased from Vital River (Beijing, China). Antagomirs of NC (5′-
CAGUACUUUUGUGUAGUACAA-3′) and miR-200c-3p (5′-
UCCAUCAUUACCCGGCAGUAUUA-3′) (10 mg kg⁻¹; GenePharma) were intraperitoneally injected into C57BL/6 mice 24 h before, and 6 and 24 h after intratracheal instillation of H5N1 virus. At 3 days postinfection, the mice were killed, and lung injury was assessed as described previously [16 (link)]. For the assessment of survival rates, virus replication and Ang II, antagomirs of NC and miR-200c-3p (5′-
UCCAUCAUUACCCGGCAGUAUUA-3′) (20 mg kg^−1; RiboBio, Guangzhou, China) were intraperitoneally injected into C57BL/6 mice 1, 24 and 48 h after intratracheal instillation of H5N1 virus. The animal experiments were performed in the Institute of Military Veterinary Medicine, Academy of Military Medical Sciences following governmental and institutional guidelines.