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Fitc labeled anti human cd68 and cd11b antibodies

Manufactured by Thermo Fisher Scientific
Sourced in China

The FITC-labeled anti-human CD68 and CD11b antibodies are laboratory reagents used for the identification and quantification of specific cell surface markers. The CD68 antibody is used to detect the CD68 protein, which is expressed on the surface of macrophages and monocytes. The CD11b antibody is used to detect the CD11b protein, which is expressed on the surface of granulocytes, monocytes, and natural killer cells. These antibodies are labeled with the fluorescent dye FITC, allowing for the detection and analysis of these cell populations using flow cytometry or other fluorescence-based techniques.

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2 protocols using fitc labeled anti human cd68 and cd11b antibodies

1

THP-1 Cell Culture and Characterization

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Human monocytic THP-1 cells were purchased from Kunming Cell Bank, Kunming Institute of Zoology, Chinese Academy of Sciences. All reagents used for cell culture including penicillin/streptomycin, FBS (fetal bovine serum) and DMEM/F12 medium were purchased from Thermo Fisher (Shanghai China). PMA was purchased from Sigma Chemical Co. (St Louis, MO). Restriction enzymes and Taq polymerase were obtained from Takara, and FITC-labeled anti-human CD68 and CD11b antibodies were purchased from Thermo Fisher (Shanghai China). RevertAid™ First Strand cDNA Synthesis Kit was from Thermo Fisher (Shanghai China). PrimeScript RT reagent kit was obtained from Takara Bio (Japan).
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2

THP-1 Cell Differentiation Assay

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Human monocytic THP-1 cells were bought from Kunming Cell Bank, Kunming Institute of Zoology, Chinese Academy of Sciences. Penicillin/streptomycin was added to all reagents used for cell culture. RevertAid™ First Strand cDNA Synthesis Kit, DMEM/F12 medium, FBS (fetal bovine serum), and FITC-labeled anti-human CD68 and CD11b antibodies were purchased from Thermo Fisher (Shanghai, China). Restriction enzymes, Taq polymerase and PrimeScript RT reagent Kit were obtained from Takara Bio (Japan). Phorbol 12-myristate 13-acetate (PMA) was purchased from Sigma Chemical Co (St Louis, USA).
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