WISP-3, VEGF-C, ERK, p38, JNK, and β-actin antibodies were obtained from GeneTex (Hsinchu, Taiwan). The phosphorylated forms of ERK, p38, and JNK antibodies were bought from Cell Signaling (Danvers, MA, USA). ERK, p38, JNK, and control ON-TARGETplus siRNAs were purchased from Dharmacon (Lafayette, CO, USA). Taqman® One-Step PCR Master Mix and qPCR primers and probes were bought from Applied Biosystems (Foster City, CA, USA). Recombinant human WISP-3 was acquired from PeproTech (Rocky Hill, NJ, USA). All other chemicals not already mentioned were acquired from Sigma-Aldrich (St. Louis, MO, USA).
Vegf c
Manufactured by GeneTex
Sourced in United States
VEGF-C is a protein that plays a key role in the regulation of angiogenesis and lymphangiogenesis. It is a member of the vascular endothelial growth factor (VEGF) family. VEGF-C is involved in the development and maintenance of blood and lymphatic vessels.
Automatically generated - may contain errors
Lab products found in correlation
Taqman one step pcr master mix, by Thermo Fisher Scientific (1 mentions)
Qpcr primers and probes, by Thermo Fisher Scientific (1 mentions)
Control on targetplus sirnas, by Horizon Discovery (1 mentions)
β actin, by GeneTex (1 mentions)
Vascular endothelial growth factor (vegf), by GeneTex (1 mentions)
Aminoethyl carbazole substrate kit, by Zymo Research (1 mentions)
Stravigen super sensitive multilink kit, by BioGenex (1 mentions)
Ki16425, by Cayman Chemical (1 mentions)
Gapdh, by GeneTex (1 mentions)
3 protocols using vegf c
1
Signaling Pathway Protein Analysis
2
Immunohistochemical Analysis of Key Proteins
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The IHC was performed according to standard procedure. Briefly, tissue sections were incubated at RT for 2 h with monoclonal antibody raised against INI1 (1:200, Santa Cruz Biotechnology, Inc., Santa Cruz, CA), VEGF (1:200, GeneTex Inc., Irvine, CA), VEGFC (1:200, GeneTex Inc., Irvine, CA), or EGFR (Santa Cruz Biotechnology, Inc., Santa Cruz, CA). The optimal dilution was determined by using human kidney as a positive control. Then, StrAviGen Super Sensitive MultiLink kit (BioGenex Laboratories, Inc., San Ramon, CA) was used to detect the resulting immune complex. Peroxidase activity was visualized using an aminoethyl carbazole substrate kit (Zymed Laboratories, Inc., San Francisco, CA).
3
Molecular Regulators of VEGF-C Signaling
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LPA was purchased from Sigma-Aldrich (Santa Cruz, CA, USA). The antibodies against VEGF-C (Genetex, Irvine, CA, USA), ATX (Abcam), CRT (Upstate Biotechnology, Lake Placid, NY, USA), and GAPDH (Genetex) were used. The inhibitors for LPA 1/3 (Ki16425), ROS (N-acetylcysteine; NAC), and ATX (S32826) were purchased from Cayman Chemical (Ann Arbor, MI, USA), Sigma-Aldrich (Santa Cruz, CA, USA) and Echelon (Salt Lake City, UT, USA), respectively. The shRNA expression vectors of LPA 1 , LPA 3 , and LEDGF were purchased from Origene Technology (Rockville, MD, USA) and CRT shRNA was acquired from the National RNAi Core Facility Platform, Academia Sinica (Taipei, Taiwan).
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