6420 triple quadrupole ms

Liquid chromatographic analyses were performed using an Agilent 1260 Infinity HPLC system (Agilent, Palo Alto, CA, USA) equipped with a binary high-pressure pump for mobile phase delivery and an autosampler. Identification and quantification of the analytes were performed on an Agilent 6420 triple quadrupole MS with electrospray source using the Agilent MassHunter Software (version B.06.00) for data analyses.
Magnetic stirrers from J.P. Selecta, S.A. (Barcelona, Spain) and a Vibramax 110 shaker from Heidolph Instruments (Schwabach, Germany) were used for the stirring during extraction and elution procedures, respectively.
Scanning electron microscope (SEM) images were obtained by using a JEOL JSM 7800F microscope (JEOL, Tokyo, Japan) at the Central Service for Research Support (SCAI) of the University of Córdoba. ATR-IR spectra were acquired with a Bruker Tensor 37 FT-IR spectrometer (Bruker Optik, GmbH, Ettlingen, Germany) equipped with a three internal reflections diamond ATR cell (Platinum ATR accessory, Bruker). Data collection and processing were done using the OPUS software package (Bruker, Ettlingen, Germany). Contact angle measurements were performed in a Ramé-hart Model 200 Standard Goniometer with DropImage Standard v2.3 equipped with an automated dispensing system at the Instituto de Ciencia Molecular (ICMol) of the University of Valencia.

Two instrumental techniques were employed in the development of the present research. The optimization of the extraction procedure and its preliminary analytical evaluation was carried out on a Waters AcquityTM Ultra Performance LC system (Waters Corp., Madrid, Spain) using an Acquity UPLC^® BEH C18 column (1.7 μm, 2.1 mm × 100 mm) working at the experimental conditions described in the Supplementary Materials. Direct infusion MS measurements were performed on Agilent 6420 Triple Quadrupole MS with electrospray source using Agilent MassHunter Software (version B.06.00, Santa Clara, CA, USA) for data analyses. The mass spectrometer settings were fixed to improve the SRM signal. The flow rate and the temperature of the drying gas (N₂) were 9 L/min and 300 °C, respectively. The nebulizer pressure was 40 psi, and the capillary voltage was kept to 2000 V in positive mode. The analytes and the internal standard were detected by Selected Reaction Monitoring (SRM) transitions, the parameters being specified in Table S1.