Allprep dna and rna isolation kit

During surgical resection of the SRS treated BM’s the center and peripheral portions of the lesion (as determined by the operating surgeon) were collected, removed from the OR, and then flash frozen using liquid nitrogen. Samples were then stored at −80 until isolation of DNA and RNA was able to be completed.
The AllPrep DNA and RNA isolation kit from Quiagen was utilized according to manufactures instructions to obtain viable DNA and RNA from patient samples. Samples were pre-minced with a tissue homogenizer and then run through a Quiagen shredder column to ensure homogenization before proceeding to AllPrep Once obtained, genomic material was QC’d and quantified using a thermo fisher Nanodrop system as well as separately QC’d by sequencing facility using Qubit and Bioanalyzer. Once DNA and RNA were obtained for patients the resulting material was stored at −80 until submission for sequencing. All patients (34) were able to provide some viable genetic material (peripheral/center DNA or RNA), however, due to the nature of the samples being dose with radiation and often necrotic isolation of high-quality RNA proved difficult for some samples, especially within the dosing center of the lesion. Control non-radiated BMs were separately obtained from the Northwestern University clinical tissue bank and were processed in the same manner as above samples.

During surgical resection of the SRS treated BM’s the center and peripheral portions of the lesion (as determined by the operating surgeon) were collected, removed from the OR, and then flash frozen using liquid nitrogen. Samples were then stored at −80 until isolation of DNA and RNA was able to be completed.
The AllPrep DNA and RNA isolation kit from Quiagen was utilized according to manufactures instructions to obtain viable DNA and RNA from patient samples. Samples were pre-minced with a tissue homogenizer and then run through a Quiagen shredder column to ensure homogenization before proceeding to AllPrep Once obtained, genomic material was QC’d and quantified using a thermo fisher Nanodrop system as well as separately QC’d by sequencing facility using Qubit and Bioanalyzer. Once DNA and RNA were obtained for patients the resulting material was stored at −80 until submission for sequencing. All patients (34) were able to provide some viable genetic material (peripheral/center DNA or RNA), however, due to the nature of the samples being dose with radiation and often necrotic isolation of high-quality RNA proved difficult for some samples, especially within the dosing center of the lesion. Control non-radiated BMs were separately obtained from the Northwestern University clinical tissue bank and were processed in the same manner as above samples.