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Rpe65

Manufactured by Merck Group
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RPE65 is a laboratory product manufactured by Merck Group. It is a protein that plays a critical role in the visual cycle, which is responsible for the conversion of light signals into electrical signals that can be processed by the visual system. The core function of RPE65 is to catalyze the isomerization of all-trans-retinyl ester to 11-cis-retinol, a key step in the visual cycle.

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Lab products found in correlation

Licor odyssey 3, by LI COR (1 mentions) Image studio lite version 5, by LI COR (1 mentions) Azure c500, by Azure Biosystems (1 mentions) Timp3, by Abcam (1 mentions) Horseradish peroxidase conjugated, by Jackson ImmunoResearch (1 mentions) Polyvinylidene difluoride membrane, by Bio-Rad (1 mentions) Synaptophysin, by Merck Group (1 mentions) Nestin, by Merck Group (1 mentions) Recoverin, by Merck Group (1 mentions) Mab1281, by Merck Group (1 mentions) Cone arrestin, by Merck Group (1 mentions) Alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Eclipse c1si, by Nikon (1 mentions) Alexa fluor 568, by Thermo Fisher Scientific (1 mentions) Parvalbumin, by Swant (1 mentions) Calbindin d 28k, by Swant (1 mentions) Collagen type 4, by Merck Group (1 mentions) Glial fibrillary acidic protein (gfap), by Merck Group (1 mentions)

2 protocols using rpe65

1

Quantitative Western Blot Analysis

Cited 1 time
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Total cell lysate and ECM were isolated and resolved by SDS-PAGE in 4% to 20% gels as previously described.17 (link) Resolved gels were transferred to polyvinylidene difluoride membranes (Bio-Rad, Hercules, CA, USA) and probed with the following primary antibodies: ACTN (1:750; Santa Cruz Biotechnology, Dallas, TX, USA), BEST1 (1:500; Millipore, Billerica, MA, USA), COL4 (1:1000; Abcam, Cambridge, MA, USA), CRALBP (1:10000),25 (link) EZR (1:1000, Cell Signaling Technology, Danvers, MA, USA), LAM (1:1000; Abcam), OCLN (1:1000; Thermo Fisher Scientific), RPE65 (1:500; Millipore), RHO (1:500; Millipore), and TIMP3 (1:250; Abcam). Secondary antibodies were host-specific near-infrared (1:12,500) (LiCor, Lincoln, NE, USA) or horseradish peroxidase conjugated (1:10,000) (Jackson ImmunoResearch, West Grove, PA, USA) and signals were detected on the LiCor Odyssey or the Azure C500 (Azure Biosystems, Dublin, CA, USA) imaging systems. After image acquisition, Western blot data were analyzed quantitatively using LiCor Odyssey 3.0 and/or Image Studio Lite version 5.2 (LiCor) and Microsoft Excel (Redmond, WA, USA).
Galloway C.A., Dalvi S., Shadforth A.M., Suzuki S., Wilson M., Kuai D., Hashim A., MacDonald L.A., Gamm D.M., Harkin D.G, & Singh R. (2018). Characterization of Human iPSC-RPE on a Prosthetic Bruch's Membrane Manufactured From Silk Fibroin. Investigative Ophthalmology & Visual Science, 59(7), 2792-2800.
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2

Immunohistochemical Profiling of Retinal Cells

Cited 1 time
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Retinal sections were stained with the following antibodies with our published protocols (34 ,35 (link)) Cone-arrestin (rabbit polyclonal, 1:1,000; Millipore), PKCα (rabbit polyclonal, 1:5,000; Sigma), recoverin (rabbit polyclonal, 1:2,000; Millipore), calbindin D28k (rabbit polyclonal, 1:1,000; Swant), neurofilament-RT97 (mouse monoclonal, 1:1,000; Millipore), parvalbumin (rabbit polyclonal, 1:3,000; Swant), synaptophysin (mouse monoclonal, 1:2,000; Millipore), RPE65 (mouse monoclonal, 1:1,000; Millipore), type IV collagen (rabbit polyclonal, 1:1,000; Millipore), MAB1281 (mouse monoclonal, 1:300; Millipore), nestin (rabbit polyclonal, 1:2,000; Millipore), GFAP (rabbit polyclonal, 1:1,000; Sigma), S100β (mouse monoclonal, 1:250; Sigma), Ki67 (rabbit polyclonal, 1:500; Millipore), TuJ1 (mouse monoclonal, 1:1,000; Sigma), STEM121 (mouse monoclonal, 1:300; StemCells). Anti-mouse or rabbit secondary antibodies conjugated to Alexa Fluor-488 or Alexa Fluor-568 (Life Technologies) were used and counterstained with 49,69-diamidino-2-phenylindole (DAPI). Images were taken with a confocal microscope (Eclipse C1si; Nikon Instruments, Inc., Melville, NY).
Tsai Y., Lu B., Bakondi B., Girman S., Sahabian A., Sareen D., Svendsen C.N, & Wang S. (2015). Human iPSC-derived neural progenitors preserve vision in an AMD-like model. Stem cells (Dayton, Ohio), 33(8), 2537-2549.
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