Lysate samples were loaded onto polyacrylamide gels and transferred to a polyvinylidene difluoride membrane (ISEQ. 00010; Millipore, Billerica, MA, USA) according to a previously reported protocol75 (link). Anti-GFP (632377; Clontech Laboratories, Mountain View, CA, USA) and anti-Penta His (34660; QIAGEN, Hilden, Germany) were used as primary antibodies, and anti-rabbit IgG (A6154; Sigma-Aldrich, St. Louis, MO, USA) and anti-mouse IgG (A4416; Sigma-Aldrich, St. Louis, MO, USA) were used as secondary antibodies.
Anti penta his 34660
Manufactured by Qiagen
Sourced in United States
Anti-penta-His (34660) is a laboratory reagent used for the detection and purification of proteins containing a histidine (His) tag. It is a monoclonal antibody that specifically binds to the His-tag sequence, enabling the identification and isolation of recombinant proteins labeled with this tag.
Automatically generated - may contain errors
Lab products found in correlation
Anti tubulin dm1a, by Merck Group (2 mentions)
Anti iga fitc c10 3, by BD (2 mentions)
Anti igg1 apc x56, by BD (2 mentions)
Anti gfp hrp b 2, by Santa Cruz Biotechnology (2 mentions)
Anti flag m2, by Merck Group (2 mentions)
Iseq00010, by Merck Group (1 mentions)
Anti mouse igg a4416, by Merck Group (1 mentions)
I5006, by Merck Group (1 mentions)
Ab1791, by Abcam (1 mentions)
3 protocols using anti penta his 34660
1
Western Blotting of GFP and His-tagged Proteins
2
Polyclonal SANBR Antibody Production
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The polyclonal SANBR antibody was produced by immunizing rabbits with the C-terminal SANBR peptide (RSKSRFGQGRPA) and isolating reactive antibodies from sera (Covance). Antibodies for flow cytometry include: anti-IgG1-APC (X56, BD Pharmingen), anti-IgA-FITC (C10-3, BD Pharmingen). Antibodies for immunoblot include: anti-AID (53 (link)), anti-tubulin (DM1A, Sigma), anti-penta-His (34660, Qiagen), anti-GFP-HRP (B-2, Santa Cruz), anti-Flag (M2, Sigma). Antibodies for chromatin immunoprecipitation (ChIP) include: anti-H3 (ab1791, Abcam) and rabbit IgG (I5006, Sigma).
3
KIAA1841 Antibody Production and Validation
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The polyclonal KIAA1841 antibody was produced by immunizing rabbits with the C-terminal KIAA1841 peptide (RSKSRFGQGRPA) and isolating reactive antibodies from sera (Covance).
Antibodies for flow cytometry include: anti-IgG1-APC (X56, BD Pharmingen), anti-IgA-FITC (C10-3, BD Pharmingen). Antibodies for immunoblot include: anti-AID 53 , anti-tubulin (DM1A, Sigma), anti-penta-His (34660, Qiagen), anti-GFP-HRP (B-2, Santa Cruz), anti-Flag (M2, Sigma).
Antibodies for flow cytometry include: anti-IgG1-APC (X56, BD Pharmingen), anti-IgA-FITC (C10-3, BD Pharmingen). Antibodies for immunoblot include: anti-AID 53 , anti-tubulin (DM1A, Sigma), anti-penta-His (34660, Qiagen), anti-GFP-HRP (B-2, Santa Cruz), anti-Flag (M2, Sigma).
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