Methylene blue dye

Manufactured by Merck Group

Sourced in Germany, United States, India, United Kingdom

Methylene blue dye is a synthetic organic compound used as a laboratory reagent. It is a blue crystalline solid that is soluble in water and alcohol. Methylene blue has a characteristic deep blue color and is commonly used as a dye, stain, and indicator in various scientific applications.

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Lab products found in correlation

42 protocols using methylene blue dye

Synthesis of Lignin Nanoparticles

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Softwood kraft lignin was obtained from Stora Enso to serve as the precursor for nanoparticle synthesis. Methylene blue dye (molecular weight: 319.85 g/mol; chemical formula: C₁₆H₁₈ClN₃S) was acquired from Sigma Aldrich (United States) and used as a model adsorbate without further purification. The LNPs were synthesized using p-toluenesulfonic acid sodium salt (PTSA) obtained from Thermo Fisher Scientific Inc. (United States) as the hydrotropic agent (C₇H₁₀O₄SNa, 213.190 g/mol, 98% purity).

Pourbaba R., Abdulkhani A., Rashidi A, & Ashori A. (2024). Lignin nanoparticles as a highly efficient adsorbent for the removal of methylene blue from aqueous media. Scientific Reports, 14, 9039.

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Hyperspectral Endoscopy of Pig Esophagus

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A fresh ex vivo pig esophagus and stomach (Medical Meat Supplies) was used as described previously^{4 (link)} to mimic the imaging conditions during endoscopy, including video rate data acquisition and data acquisition in a narrow lumen. Hyperspectral endoscopy was performed using a grating of 50 lines∕mm and exposure time of 25 ms, with motion from right to left sides of the lumen used to build up the second spatial dimension of information. Methylene blue dye (319112, Sigma-Aldrich) was sprayed inside of the lumen to induce a color change for identification with our color-based classification approach.

Grigoroiu A., Yoon J, & Bohndiek S.E. (2020). Deep learning applied to hyperspectral endoscopy for online spectral classification. Scientific Reports, 10, 3947.

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Synthesis of Photocatalytic Nanocomposites

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The chemicals such as zinc nitrate hexahydrate (Zn(NO₃)₂·6H₂O), silver Nitrate (AgNO₃), Methylene Blue dye (MB), were purchased from Sigma-Aldrich-Germany and used as received. The glassware was used with analytical boro-silicate grade.

Alharthi F.A., Alghamdi A.A., Al-Zaqri N., Alanazi H.S., Alsyahi A.A., Marghany A.E, & Ahmad N. (2020). Facile one-pot green synthesis of Ag–ZnO Nanocomposites using potato peeland their Ag concentration dependent photocatalytic properties. Scientific Reports, 10, 20229.

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Photocatalytic Decolorization of Methylene Blue

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The following reagents were used: Titanium (IV) oxide from Sigma-Aldrich (718467-100G, St. Louis, MO, USA); nanopowder Aeroxide^® P25 of Ebonik Degussa, known as Degussa P25, with an average size of 21 nm, purity

\geq

99.5%, and Lot. # MKBC4174; sodium borohydride NaBH₄ from Aldrich (452874-25G, St. Louis, MO, USA), and Lot. # MKBZ2657V; isopropyl alcohol from J. T. Baker (9084-02, Phillipsburg, NJ, USA); Baker analyzed ACS; absolute ethanol, J. T. Baker; reactive Baker ACS (Cat. # 9000-03, USA), and Lot. # V32C55. For the photocatalytic test, deionized water with a resistivity of 18.2 MΩ cm and methylene blue dye from Sigma (M9140-25G, St. Louis, MO, USA) were used.

Sabinas-Hernández S.A., Gracia Jiménez J.M., Silva González N.R., Elizalde-González M.P., Salazar-Kuri U, & Tehuacanero-Cuapa S. (2022). Blue Titania: The Outcome of Defects, Crystalline-Disordered Core-Shell Structure, and Hydrophilicity Change. Nanomaterials, 12(9), 1501.

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Adsorption of Methylene Blue on Aluminosilicate

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SS-100 mesh (316 L), NaAlSi₂O₆–H₂O (99.9% purity), and methylene blue dye were purchased from Sigma-Aldrich (St. Louis, MO, USA). Double distilled deionized (DI) water was used throughout the study.

Ahmad S., Egilmez M., Iqbal M., Ibrahim T., Khamis M, & Alnaser A.S. (2021). Pulsed Laser Deposited Zeolite Coatings on Femtosecond Laser-Nanostructured Steel Meshes for Durable Superhydrophilic/Oleophobic Functionalities. Frontiers in Chemistry, 9, 792641.

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Lateral Ventricle Dye Infusion

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Following testing, methylene blue dye (Sigma-Aldrich Co. LLC, St. Louis, MO, USA) was infused through the internal cannula, and animals were euthanized with CO₂ inhalation and decapitated. Brains were immediately removed and frozen at −80°C. Brains were sliced in 40-μm sections on a cryostat vibratome (Leica Microsystems Inc., Bannockburn, IL), and infusion locations were verified within the lateral ventricle, according to the Paxinos and Watson (1998) rat brain atlas. Data from animals exhibiting surgical placements in areas outside of the lateral ventricle were excluded.

Franklin K.M., Hauser S.R., Lasek A.W., Bell R.L, & McBride W.J. (2015). Involvement of purinergic P2X4 receptors in alcohol intake of high-alcohol-drinking (HAD) rats. Alcoholism, clinical and experimental research, 39(10), 2022-2031.

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Bread with Nitrate Carbonization

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The following ingredients were used: flour (300 g), yeast (5 g), water (400 mL), Iron nitrate crystals (4 mg), deionised water.
Iron nitrate of analytical grade purity and methylene blue dye were purchased from Sigma-Aldrich. The argon gas used for the carbonisation process was purchased from Afrox.

Khumalo S.P., Lokhat D., Chetty K, & Chetty L. (2022). Synthesis of novel carbon-supported iron oxide sorbents for adsorption of dye from aqueous solutions: equilibrium and flow-through studies. Scientific Reports, 12, 20009.

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Synthesis and Characterization of ZnO Nanoparticles

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Zinc acetate (Zn(CH₃COO)₂‧2H₂O) was used as a precursor for ZnO-NPs, and it was purchased from Sigma-Aldrich (Cairo, Egypt), possessing a purity percentage of 99%. The components of media used in the current study, as well as methylene blue dye, were obtained from Sigma-Aldrich (Taufkirchen, Germany), with purity percentages in the ranges of 95–99%. All reactions were achieved using distilled water (dH₂O). The bacterial and fungal strains used in antimicrobial activity were obtained from the Microbial Physiology Lab., Faculty of Science, Al-Azhar University, Cairo, Egypt.

Fouda A., Saied E., Eid A.M., Kouadri F., Alemam A.M., Hamza M.F., Alharbi M., Elkelish A, & Hassan S.E. (2023). Green Synthesis of Zinc Oxide Nanoparticles Using an Aqueous Extract of Punica granatum for Antimicrobial and Catalytic Activity. Journal of Functional Biomaterials, 14(4), 205.

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Verifying Injection Locations in Rat Brains

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To verify injection location, rats were injected with 0.3 μl methylene blue dye (Sigma–Aldrich), anesthetized with ketamine and xylazine (100 mg/kg and 13 mg/kg, i.p.), and decapitated. Brains were post-fixed in 4% paraformaldehyde for 72 hours at 4 °C, transferred to 25% sucrose for 3-4 days at 4 °C, and then frozen at −80 °C. They were cut in coronal 30 μm sections and slide-mounted for microscopic examination. One animal in the aPVT group and two from the pPVT group had a probe more than 0.5 mm from the target region, so data from these rats were discarded. One additional rat in the aPVT group began to exhibit poor health during the study and was removed prior to EPM testing.

Barson J.R, & Leibowitz S.F. (2015). GABA-induced inactivation of dorsal midline thalamic subregions has distinct effects on emotional behaviors. Neuroscience letters, 609, 92-96.

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Synthesis of Metal Sulfates and Chromates

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All the chemicals (MnSO₄·H₂O, K₂Cr₂O₇, HCl, NaOH, DMSO, methylene blue dye, nutrient agar, nutrient broth, ethanol, and methanol) of analytic grade were purcased from Sigma-Aldrich Darmstadt, Germany.

Khan S.A., Shahid S., Shahid B., Fatima U, & Abbasi S.A. (2020). Green Synthesis of MnO Nanoparticles Using Abutilon indicum Leaf Extract for Biological, Photocatalytic, and Adsorption Activities. Biomolecules, 10(5), 785.

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