Deadend fluorometric tunel assay kit

PTX was purchased from
Lc Laboratories (Wobum, MA). PTX–VE and 5-FU–TPGS were
synthesized in our lab (Figure 2). The synthesis
and characterization of PTX–VE and 5-FU–TPGS is provided
in the Supporting Information (Figures
S1–S4). Paclitaxel injection (Taxol) was manufactured by Ben
Venue Laboratories, Inc. (Bedford, OH). 5-Fluorouracil injection
was purchased from APP Pharmaceuticals, LLC (Schaumburg, IL). Antibodies
against p-53, β-tubulin, P-gp, GAPDH, and horseradish peroxidase
(HRP)-conjugated anti-mouse or anti-rabbit whole IgG were obtained
from Santa Cruz Biotechnology (San Diego, CA). DeadEnd Fluorometric
TUNEL assay kits were obtained from Promega (Madison, WI). Other chemicals
were purchased from Sigma-Aldrich (St. Louis, MO).
KB-3-1 and KB-8-5 cells originally obtained from American
Type
Culture Collection (ATCC) (Manassas, VA) were maintained in RPMI 1640
and DMEM medium (Invitrogen, Carlsbad, CA) containing 10% fetal bovine
serum (Invitrogen, Carlsbad, CA), 100 unit/mL penicillin, and 100
μg/mL streptomycin (Invitrogen, Carlsbad, CA). Cells were cultivated
in a humidified incubator at 37 °C and 5% CO₂.
Mice were purchased from the National Cancer Institute (Bethesda,
MD). All experiments performed on animals were in accordance with
and approved by the Institutional Animal Care and Use Committee at
the University of North Carolina at Chapel Hill.