The surface morphology of MYR, excipients, and MyNF were observed by scanning electron microscopy (SEM; Hitachi S4700, Hitachi, Tokyo, Japan). Each sample was sputter-coated with a thin gold layer using an ion sputter coater (Hitachi E-1045, Hitachi, Tokyo, Japan) before SEM analysis. The particle size of MYR and each MyNF was measured by a dynamic light scattering particle size analyzer (DLS; ELSZ-2000; Otsuka Electronics, Osaka, Japan). The sample preparation for particle size measurement involved dissolving each formulation (containing 1 mg MYR) in 1 mL distilled water and then diluted it with 10-fold pure water and immediately placed in a cuvette for determining the particle size. Additionally, the particle morphology of MyNF was observed using a transmission electron microscope (TEM; JEOL JEM-2000 EXII; JEOL, Tokyo, Japan). MyNF (containing 1 mg MYR) was dissolved in 1 mL distilled water and diluted 100-fold with distilled water, and then dropped into the carbon-coated copper grid and stained with 0.5% (w/v) phosphotungstic acid (Sigma, St. Louis, MO, USA). Each sample was kept in a moisture-proof container until TEM observation.
Jem 2000exii
Manufactured by JEOL
Sourced in Japan, United States
The JEM-2000EXII is a High-Resolution Transmission Electron Microscope (HR-TEM) designed and manufactured by JEOL. It is capable of producing high-quality images and diffraction patterns of samples at the nanometer scale. The microscope's core function is to enable detailed structural and compositional analysis of materials using electron beam interaction with the specimen.
Automatically generated - may contain errors
Lab products found in correlation
Phosphotungstic acid, by Merck Group (4 mentions)
Zetasizer nano zs90, by Malvern Panalytical (3 mentions)
S 4700, by Hitachi (2 mentions)
Fcf 200 cu, by Electron Microscopy Sciences (2 mentions)
V 650, by Jasco (2 mentions)
E 1045, by Hitachi (1 mentions)
Zetasizer 3000hs, by Malvern Panalytical (1 mentions)
Zetasizer 3000hs analyzer, by Malvern Panalytical (1 mentions)
Zetasizer 3000hsa, by Malvern Panalytical (1 mentions)
Kmno4, by Fujifilm (1 mentions)
Smartlab, by Rigaku (1 mentions)
Em uc6, by Leica (1 mentions)
S 4000, by Hitachi (1 mentions)
Uranyl acetate, by Fujifilm (1 mentions)
Planapon60xosc na1, by Olympus (1 mentions)
D2 phaser powder x ray diffractometer, by Bruker (1 mentions)
Ft 730 ftir spectrometer, by Horiba (1 mentions)
H 7500, by Hitachi (1 mentions)
Carbon coated 200 mesh copper specimen grid, by Agar Scientific (1 mentions)
Jc 1 dye, by Thermo Fisher Scientific (1 mentions)
58 protocols using jem 2000exii
1
Morphological Characterization of MYR and MyNF
2
Electron Diffraction of Crystalline Samples
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The electron diffraction patterns were taken with a JEOL JEM-2000EXII (JEOL, Japan) at an accelerating voltage of 100 kV. A drop of the dispersion of crystals was dried on a carbon-coated Cu grid. The electron diffraction patterns were obtained in the microdiffraction mode. The distance in the diffraction patterns was calibrated with the (111) diffraction ring of evaporated Au particles.
3
Ultrastructural Analysis of Mechanically Strained VSMCs
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VSMCs in collagen I-coated BioFlex® plates were subjected to mechanical strain and MNPs, gently rinsed with PBS, and fixed with 4% paraformaldehyde. After fixation, cells were thoroughly rinsed with ddH2O, followed by treatment with 1% osmium tetroxide with shaking at 5 rpm for 1 h. The samples were dehydrated with graded ethanol and embedded in Epon resin, with the silicon membrane carefully removed from the resin. Sections with 80 nm thickness were obtained, counterstained with 4% uranyl acetate in hydrogen peroxide for 2 h and 0.4% lead citrate for 10 min, and examined with TEM (Jeol JEM-2000 EXII; JEOL USA, Inc., MA, USA). Image processing and analysis of intracellular vesicles were performed using Image J software.
4
Ultrastructural Analysis of Ginseng Leaves
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The ginseng leaves were also fixed in 4% (v/v) glutaraldehyde in 25mM phosphate buffer (pH 7.1) for 2 hours, and postfixed in 2% (w/v) osmium tetroxide solution in same buffer for 1 hour. Dehydration was accomplished in a graded ethanol series. The samples were placed in propylene oxide prior to further treatment, and then embedded in a Spurr mixture. Semithin sections were cut with a glass knife and ultramicrotome (Reichert Ultracut S, Vienna, Austria). They were then stained with toluidine blue and basic fuchin for preliminary screening with a light microscope. Ultrathin sections approximately 70 nm thick were cut with a diamond knife, and then stained on copper grids with 1% (w/v) uranyl acetate and lead citrate [21] (link). They were examined with a transmission electron microscope (JEM 2000 EX II; JEOL Ltd.) operated at 80 kV.
5
Characterization of ZnFe2O4 Nanoparticles
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The particle size and particle size distribution as well as the morphology of nanoparticles were evaluated by transmission electron microscopy (TEM) (JEM-2000 EX II; JEOL, Tokyo, Japan). Magnetization properties were measured by vibrating Sample Magnetometer (VSM 7400 Lake Shore) and X-ray diffraction (The D8 ADVANCE X-ray Spectrometer, a Copper X-ray tube operated at 40 kV and 40 mA, manufactured by Brucker Co.) technique to identify the crystalline phases. Moreover, size distribution of ZnFe2O4 NPs in suspension (normal saline) was performed using Dynamic Light Scattering (DLS) and Zeta potential using Zetasizer 3000HS (Malvern Instruments, Malvern, Worcestershire, UK).
6
Nanofibrous Morphology Characterization
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Different samples of nanofibers were plated with platinum with an ion coater (E-1045, HITACH, Tokyo, Japan); the condition was set at 10 mA 120 s later. The morphology and shape of each sample was observed by a scanning electron microscope (Hitachi S4700, Hitachi, Tokyo, Japan). The diameter of each sample was calculated by image j software. A Zetasizer 3000HS analyzer (Malvern, Worcestershire, UK) was used to measure the particle size of OAnf. The particle size of OA and OAnf were measured at a concentration of 1 mg/mL and 0.1 mg/mL, respectively. In addition, we also observed the uniformity of the morphology of OAnf after dissolving in water using a transmission electron microscope (TEM, JEM-2000EXII instrument, JEOL Co., Tokyo, Japan). The test sample was adjusted to 1 µg/mL of OA in deionized water and then dripped into the copper mesh, and then 0.5% (w/v) phosphotungstic acid was immediately dripped. After drying, each sample was placed on the TEM for observation.
7
Polymer-Based FAK siRNA Delivery
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The various moles of the mPEG-b-P(HEMA-ketal-VB6) were dissolved in 2 mL of dimethyl sulfoxide (DMSO) and mixed with 1 mL of PBS. Afterwards, various concentrations of the polymer solutions were blended with 0.5 mL of the FAK siRNA solution (1 µg/mL) and incubated at 25 °C. At 3–4, 8–9, and 24 h post-incubation, the polyplex solutions were independently placed into a dialysis bag (M.W.C.O. 6–8 k) and dialyzed against deionized water. After dialysis, the solutions were placed in an ultracentrifuge filter tube (M.W.C.O. 10 k) and the volumes of the solutions were concentrated until reaching 3.5 mL. The polyplex samples were analyzed with agarose gel electrophoresis and measured within the UV-vis spectrum. Additionally, the particle sizes of the polyplexes were measured with dynamic light scattering (DLS) (Zetasizer 3000HSA, Malvern Panalytical, Worcestershire, UK). The particle sizes were analyzed using CONTIN method. The morphologies of the polyplexes at pH 7.4 and pH 5.0 were observed using transmission electron microscopy (TEM) (JEM-2000 EXII, JEOL Ltd., Tokyo, Japan) after staining with the 2% uranyl acetate.
8
Negative Staining Protocol for Liposomal Morin
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TEM is the most frequently used imaging technique for characterizing morphology of nano-scaled particles such as liposomes. In this study, we prepared samples by the negative-staining method due to its simplicity and availability. Each sample of liposomal morin formulations was completely dissolved in de-ionized distilled water to concentration 100 µg/mL. Then 200 µL of prepared solution was slowly dripped onto a 200-mesh copper grid and left to dry. At the same time, phosphotungstic acid (Sigma, St Louis, MO, USA) 0.5% (w/v) in distilled water was made ready to drop onto dried vesicles (200 µL was used for each copper grid). The resulting samples were stored in the empty plastic capsules until being placed in a transmission electron microscope (JEM-2000EXII instrument; JEOL Co., Tokyo, Japan) for their morphological observation.
9
Polyplex Characterization by TEM
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Polyplexes were prepared with various N/P ratios of CS-PEI and siRNA using an equal volume of 250 μL serum-free medium containing various amounts of CS-PEI to adjust an N/P ratio with a fixed amount of 100 pmol siRNA. Polyplexes were formed for 30 min at room temperature. The size and morphology of polyplexes were observed by TEM (JEM-2000 EXII; JEOL, Tokyo, Japan). TEM samples were prepared as previously reported.37 (link)
10
Aβ Peptide Morphology with Cu2+ Ions
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A transmission electron microscopy (JEM-2000 EXII, JEOL, Japan) with an accelerating voltage of 100 KeV was used to analyze the morphology of Aβ peptides incubated with Cu2+. Ten microliters of sample with the different Aβ peptides and Cu2+ ions in 1∶1 molar ratio used for the aggregation assay was used. Each peptide sample was placed onto a carbon-coated 200 mesh copper grid (Pelco, Ca, USA). Excess solution was wicked dry with tissue paper, and the sample was negatively stained with 5 ml of 2% uranyl acetate for 30 seconds. After TEM analyses, these copper grids coated with Aβ samples used for TEM analyses were further treated with 50 μL 1 mM EDTA solution three times to strip off Cu2+ ions and then incubated at 37°C for 24 hrs. These copper grids coated with Aβ samples treated with EDTA were then conducted for TEM analyses to observe the morphology of Aβ peptides in absence of Cu2+.
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