Ethanol (for spectroscopy; Uvasol, purity ≥ 99.9%) and ethyl acetate (for spectroscopy; Uvasol, purity ≥ 99.9%) were purchased from Merck KGaA (Darmstadt, Germany). Nile red (technical grade) was purchased from Sigma-Aldrich Chemie GmbH (Steinheim, Germany). Water was Milli-Q quality with an organic total mass of 2 ppb (parts per billion) and resistivity of 18.2 MΩ/cm. All chemicals were used without further purification. The literature values and measured values of the refractive indices, densities, and viscosities of the 3 pure components are presented in Table S2 . Figs. S8 and S9 show the literature densities and refractive index map of the ternary system at 25 °C, and Fig. S10 shows the modeled refractive index map. The description of the used software and fitting parameters needed for the calculations and visualization of the refractive index map can be found in the Supplementary Information and Table S3 . The relative errors of the calculated refractive index at 25 °C compared to the combined experimental values of Andrade et al. [20 ] and Robles et al. [21 ] are shown in Fig. S11 . These errors are in the fourth digit of the refractive index.
Uvasol
Manufactured by Merck Group
Sourced in Germany
Uvasol is a laboratory equipment product manufactured by Merck Group. It is a specialized device used for spectroscopic analysis. The core function of Uvasol is to measure the absorption or transmission of light through a sample in the ultraviolet and visible light spectrum.
Automatically generated - may contain errors
Lab products found in correlation
Lichrosolv, by Merck Group (5 mentions)
Acetone, by Merck Group (3 mentions)
Fluorolog 3, by Horiba (2 mentions)
P nitrophenyl phosphate pnpp, by Merck Group (2 mentions)
Milli q equipment, by Merck Group (2 mentions)
N n methylenebis acrylamide mba, by Merck Group (2 mentions)
Hypericin, by Merck Group (2 mentions)
Chromasolv, by Merck Group (2 mentions)
Nile red technical grade, by Merck Group (1 mentions)
Ethanol, by Merck Group (1 mentions)
Acrylamide, by Bio-Rad (1 mentions)
Oligonucleotides, by Eurofins (1 mentions)
Lambda 950 uv vis nir spectrophotometer, by PerkinElmer (1 mentions)
Lambda 35 spectrophotometer, by PerkinElmer (1 mentions)
Nicolet 6700 ft ir spectrometer, by Thermo Fisher Scientific (1 mentions)
Vnmrs 600 mhz spectrometer, by Agilent Technologies (1 mentions)
Hp 8452a, by Hewlett-Packard (1 mentions)
Dodecanethiol, by Merck Group (1 mentions)
Hexadecanethiol, by Merck Group (1 mentions)
Ellman s reagent 5 5 dithiobis 2 nitrobenzoic acid, by Merck Group (1 mentions)
49 protocols using uvasol
1
Ternary System Refractive Index Mapping
2
High-Pressure Nanostructure Synthesis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Nanostructured
systems have been prepared in a homemade, high-pressure visual variable
volume cell (HPVVVC), which is described in theSI (Figure S1). acetone (Uvasol) and acetonitrile (LiChrosolv)
purchased from Merck Millipore (Germany) both with a purity of 99.9%
as well as CO2 4.5 purchased from Linde (Germany) with
a purity of 99.995% were used without further purification. Water
was highly purified (conductivity <18 μS cm–1) in house by an ion-exchange water system (SG 2800 SK, SG Wasseraufbereitung
and Regenerierstation, Germany). For hp-SANS measurements, acetone
(Uvasol) has been replaced by its perdeuterated form (99.9 atom %
D) purchased from Sigma-Aldrich and used without further purification.
systems have been prepared in a homemade, high-pressure visual variable
volume cell (HPVVVC), which is described in the
purchased from Merck Millipore (Germany) both with a purity of 99.9%
as well as CO2 4.5 purchased from Linde (Germany) with
a purity of 99.995% were used without further purification. Water
was highly purified (conductivity <18 μS cm–1) in house by an ion-exchange water system (SG 2800 SK, SG Wasseraufbereitung
and Regenerierstation, Germany). For hp-SANS measurements, acetone
(Uvasol) has been replaced by its perdeuterated form (99.9 atom %
D) purchased from Sigma-Aldrich and used without further purification.
3
MALDI-TOF Mass Spectrometry Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Acetone (Uvasol®), trifluoroacetic acid (Uvasol®), and 2,5-dihydroxybenzoic acid (DHB) were purchased from Merck KGaA (Darmstadt, Germany). Water (HPLC grade) was purchased from Sigma (Steinheim, Germany). Gelatine was purchased from VWR Chemicals (Leuven, Belgium).
4
Synthesis and Characterization of Nucleoside Analogues
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
1-Methyl-2(1H)-pyrimidinone (Specs), β-D-ribose (97%, Sigma-Aldrich) and zebularine (≥98%, Sigma-Aldrich) were used as received. The solvents water (Chromanorm, VWR chemicals), methanol (Uvasol, Merck KGaA), perdeuterated methanol (Euriso-top, 99.8%), and tetrahydrofuran (Uvasol, Merck KGaA) were of spectroscopic grade. Research grade gases (nitrogen (N50), oxygen (N48), and nitrous oxide (N25)) were supplied by Air Liquide.
5
Protein Purification and Molecular Cloning
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All chemicals were purchased from Merck KGaA, (Darmstadt, Germany), and were of analytical grade, except for methanol used to dilute odorants, which was of spectroscopic grade (Uvasol). Acrylamide and affinity columns for His-tag protein purification were from Bio-Rad, Vienna, Austria. Long-chain unsaturated aldehydes and alcohol were from Bedoukian (Danbury, CT, USA). Oligonucleotides were custom synthesized at Eurofins Genomics (Ebersberg, Germany). All enzymes and kits for DNA purification were from New England Biolabs (Ipswich, MA, USA).
6
Characterization of Luminescent Materials
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
UV-vis absorption spectra were recorded on a Probe Drum Lab-in-a-box spectrometer. Steady-state emission measurements were performed on a Fluorolog-3 (Horiba) fluorimeter. Emission spectra were background subtracted and corrected for the wavelength dependent instrument response.
All samples were prepared in a solvent mixture of acetonitrile (spectroscopic grade Uvasol®, ≥99.9%, Merck) and methanol (ACS spectroscopic grade, ≥99.9%, Merck) (volume ratio 4 : 3) or in neat acetonitrile with absorptions of around 0.0755 ± 0.005 at the excitation wavelength (502 or 525 nm).
All samples were prepared in a solvent mixture of acetonitrile (spectroscopic grade Uvasol®, ≥99.9%, Merck) and methanol (ACS spectroscopic grade, ≥99.9%, Merck) (volume ratio 4 : 3) or in neat acetonitrile with absorptions of around 0.0755 ± 0.005 at the excitation wavelength (502 or 525 nm).
7
HPLC Assay for Preservatives in HPV-2 Vaccines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A high-performance liquid chromatography (HPLC) assay was used to determine the content of preservatives in the HPV-2 vaccines according to the standard curve. The chromatographic conditions were as follows: a Waters Acquity Peptide BEH C18 300 A (150 mm × 2.1 mm, 1.7 μm) column was selected; mobile phases were A = 0.1% trifluoroacetic acid (Merck UVASOL) aqueous solution, B = 0.1% trifluoroacetic acid acetonitrile (J.T. Baker) solution, and the elution gradient is shown in Table 2 ; the flow rate was 0.2 ml/min; the column temperature is 40°C the sample tray temperature was 12°C and the detection wavelength was 254 nm.
Elution gradient of preservative content detected by HPLC.
| retention time (min) | A | B |
|---|---|---|
| (0.1% TFA in H2O, %) | (0.1% TFA in ACN, %) | |
| 0 | 90 | 10 |
| 4 | 90 | 10 |
| 20 | 50 | 50 |
| 22 | 20 | 80 |
| 25 | 90 | 10 |
| 35 | 90 | 10 |
TFA: trifluoroacetic acid; ACN: acetonitrile.
8
Spectroscopic Analysis of Aligned PBI Films
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
UV–vis absorption spectra in solution were recorded using a Perkin Elmer Lambda 35 spectrophotometer. The samples were prepared in spectroscopic grade solvent (Uvasol®, Merck, Hoehenbrunn, Germany) and quartz glass cuvettes were used. Extinction coefficients were calculated from Lambert–Beer’s Law.
The solid-state studies were recorded with a Perkin Elmer Lambda 950 UV–Vis–NIR spectrophotometer equipped with an integrating sphere. The spectra were obtained in reflection mode. The samples were coated on Ulland quartz glass plates. For the polarized UV–vis measurements, a polarizer was inserted in between the light source and the sample. The aligned samples for polarized UV–vis measurements, were prepared on quartz glass substrates that were successively cleaned with chloroform, toluene, isopropanol, and acetone and heated to 70 °C. Then, 10 µL PBI solution (10−4 to 10−3 M in MCH) was placed with an Eppendorf pipette between the substrate and the shearing plate (tilted angle = 15°) in a home-made shearing device and the solution was sheared at 0.083 mm s−1 at 70 °C. The dichroic ratio of the aligned samples was calculated according to literature52 (link).
The solid-state studies were recorded with a Perkin Elmer Lambda 950 UV–Vis–NIR spectrophotometer equipped with an integrating sphere. The spectra were obtained in reflection mode. The samples were coated on Ulland quartz glass plates. For the polarized UV–vis measurements, a polarizer was inserted in between the light source and the sample. The aligned samples for polarized UV–vis measurements, were prepared on quartz glass substrates that were successively cleaned with chloroform, toluene, isopropanol, and acetone and heated to 70 °C. Then, 10 µL PBI solution (10−4 to 10−3 M in MCH) was placed with an Eppendorf pipette between the substrate and the shearing plate (tilted angle = 15°) in a home-made shearing device and the solution was sheared at 0.083 mm s−1 at 70 °C. The dichroic ratio of the aligned samples was calculated according to literature52 (link).
9
FTIR Analysis of Solid Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For chemical analysis, FTIR was used. 1 wt % of the sample to be analyzed was mixed with KBr (potassium bromide for IR spectroscopy Uvasol®, Merck Millipore, Darmstadt, Germany) and pressed into a pellet. The spectra were recorded in absorbance mode (Nicolet 6700 FTIR spectrometer, Thermo Scientific, Waltham, MA, USA) and collected between 4000 and 400 cm−1. Pure KBr was used to correct the background noise.
10
Spectroscopic analysis of organic compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
NMR spectra were recorded in 5 mm NMR tubes on a VNMRS 600 MHz spectrometer (600 MHz for 1H; 150 MHz for 13C, Varian, Inc., Palo Alto, CA, USA) in DMSO-d6 and CDCl3 as solvents. Chemical shifts are referenced to tetramethylsilane (TMS) as an internal standard. Attenuated total reflection Fourier transform infrared (ATR-FTIR) spectra of all the described experiments were measured on a Nicolet 6700 FTIR (from ThermoNicolet Corp., Madison, WI, USA). Spectra were recorded with ATR mathematical corrections yielding a 1.0 cm−1 actual resolution and 40 measurements were averaged. Electronic absorption spectra were obtained on an HP 8452A diode array spectrophotometer (Hewlett Packard, Palo Alto, CA, USA). The solvents used (CHCl3 and DMF) were HPLC (CHCl3; LiChrosolv®, Merck, Darmstadt, Germany) or UV-spectroscopy grade (DMF; Uvasol®, Merck) and were used without further purification.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!