Gapdh bsm 33033m

Manufactured by Bioss Antibodies

Sourced in China, United States

GAPDH (bsm-33033M) is a laboratory antibody product manufactured by Bioss Antibodies. It is a primary antibody directed against the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein. GAPDH is a ubiquitous enzyme involved in the glycolytic pathway.

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GAPDH (93 citations) Bsm-33033M (6 citations)

2 protocols using gapdh bsm 33033m

Osteosarcoma Cell Lines and Antibody Specifications

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The human osteosarcoma cell lines MG63, MNNG-HOS and Saos-2 were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). The human osteosarcoma cell line U-2OS was purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). These cell lines were cultured following the ATCC protocols. Standardized culture conditions had been described previously [18 (link)].
The antibodies used were S1PR3 (ab126622; Abcam, Cambridge, UK), YAP (ab52771; Abcam, Cambridge, UK), p-YAP (ab76252; Abcam, Cambridge, UK), c-Myc (ab32072; Abcam), Ki67 (GB13030; Servicebio, Wuhan, China), β-actin (M1210-2; Hua'an Biology, Chuzhou, China), GAPDH (bsm-33033M; Bioss, Beijing, China), anti-rabbit IgG light chain (ab99697, Abcam), anti-rabbit IgG heavy chain (ab99702, Abcam), and anti-mouse IgG light chain (A25012, Abbkine, CA, USA).

Shen Y., Zhao S., Wang S., Pan X., Zhang Y., Xu J., Jiang Y., Li H., Zhang Q., Gao J., Yang Q., Zhou Y., Jiang S., Yang H., Zhang Z., Zhang R., Li J, & Zhou D. (2018). S1P/S1PR3 axis promotes aerobic glycolysis by YAP/c-MYC/PGAM1 axis in osteosarcoma. EBioMedicine, 40, 210-223.

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Western Blot Analysis of Protein Expression

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The collected cells were washed with pre-cooled PBS, lysed with a mixture of RIPA lysis buffer and protease inhibitor cocktail (HY-K0010, MedChemExpress, USA) for 10 min at room temperature, and then centrifuged at 10,000 rpm for 10 min at 4 °C. Denaturing was performed with 5 × SDS–PAGE loading buffer at 100 °C for 5 min. After SDS–PAGE, the samples were transferred to a PVDF membrane (Millipore, ISED00010). Blocking was performed with 5% skimmed milk for 2 h at room temperature, and the blocked membranes were incubated with primary antibodies (H3K27me3, 17-622, Millipore, USA; NP, ab104870, Abcam, USA; HSP90, 60318-1-Ig, Proteintech Group, USA; GAPDH, bsm-33033M, Bioss, UK) overnight at 4 °C. After the membranes were washed with PBST, they were incubated with a secondary antibody (SA00001-1, Proteintech Group, USA) for 1 h at room temperature, washed with PBST again, and then exposed on the chemiluminescence imager.

Zhang L., Jin J., Qin W., Jiang J., Bao W, & Sun M.A. (2023). Inhibition of EZH2 Causes Retrotransposon Derepression and Immune Activation in Porcine Lung Alveolar Macrophages. International Journal of Molecular Sciences, 24(3), 2394.

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