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Normal human plasma

Manufactured by Merck Group
Sourced in United States

Normal human plasma is a common laboratory product used as a reference material in various tests and analyses. It is a cell-free liquid component of human blood that contains a variety of proteins, electrolytes, and other components. Normal human plasma serves as a baseline for comparison and standardization purposes in clinical and research settings.

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2 protocols using normal human plasma

1

Isolation and Culture of DCs from PBMCs

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Peripheral blood mononuclear cells (PBMCs) were isolated from heparinized blood (Biology Speciality Corporation) by Ficoll-Paque Plus (Amersham Biosciences) density gradient centrifugation. Monocytes obtained by the adherence method from PBMCs were cultured in 1% normal human plasma (Sigma-Aldrich) in the presence of rhGM-CSF (100 IU/ml; PeproTech) and rhIL-4 (300 IU/ml; PeproTech) for 5 days. Cells were provided with fresh cytokines every other day. pDCs and mDCs isolation from PBMCs was carried out by magnetic separation with a MACS Separator (Miltenyi Biotec) according to manufacturer’s protocol.
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2

Plasma-based HIV Treatment Protocol

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Normal human plasma was purchased from Sigma-Aldrich, St. Louis, MO, USA (Cat # H4522). HIV-infected and cART-treated (Prezcobix 800 mg-150 mg, Tivicay 50 mg, Etravirin 200 mg) patient plasma was purchased from Innovative Research (Peary Court, Novil, MI, USA).
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