Samples were generated by incubating 1% NHS with 400 μg/ml heat-aggregated IgG in GVBS++ at each temperature for one hour. Heat-aggregated IgG was generated from purified human IgG (Gammagard, Baxter) as described elsewhere [23 (link)]. The reaction was stopped with EDTA-GVBS- - and the samples were measured by C5a ELISA (R&D Systems). Background values from controls of NHS and heat-aggregated human IgG incubated on ice for one hour were subtracted.
C5a elisa
Manufactured by R&D Systems
The C5a ELISA is a quantitative sandwich enzyme-linked immunosorbent assay designed for the measurement of human C5a in biological samples such as cell culture supernatants, serum, and plasma.
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Lab products found in correlation
2 protocols using c5a elisa
1
C5a ELISA Assay for Complement Activation
2
Anti-GD2 Antibody ELISA for C5a Quantification
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MaxiSorp 96 well ELISA plates (NUNC) were coated with 10 ug/ul anti-GD2 antibody overnight at 4°C. Afterwards, plates were washed and pooled human serum was added for 15 min or 60 min at 37°C. Subsequently C5a was quantified by performing a C5a ELISA (R&D systems) according to the manufacturers protocol.
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