1 fluoro 2 4 dinitrobenzene

Manufactured by Merck Group

Sourced in Japan, Poland, United States

1-Fluoro-2,4-dinitrobenzene is a chemical compound used in various laboratory applications. It is a yellow crystalline solid with the molecular formula C₆H₃FN₂O₂. The compound is commonly used as a reagent in organic synthesis and analytical chemistry.

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4-dinitrobenzene (8 citations)

6 protocols using 1 fluoro 2 4 dinitrobenzene

Synthesis and Characterization of Lipid Nanoparticles

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N-Fmoc-amido-dPEG₆-acid [Fmoc is also known as fluoren-9-ylmethoxycarbony] was purchased from Quanta BioDesign. N-Fmoc-Glu(OtBu)-OH, Boc-Lys(Fmoc)-OH, Fmoc-lys(ivDde)-OH, NovaPEG Rink Amide resin, HBTU [2-(1H-benzotriazol-1-yl)-1,1,3,3-tetramethyluroniumhexafluorophosphate], Fmoc-Arg(pfb)-OH and BSA (Bovine Serum Albumin) was purchased from EMD Biosciences. IgE^DNP (clone SPE-7), dansyl chloride, 1-Fluoro-2,4-dinitrobenzene (DNFB), DIEA (N,N-diisopropylethylamine), TFA (trifluoroacetic acid), Triisopropylsilane (TIS), hydrazine, Cholesterol, Dichloromethane, 2-proponol, ACN(acetonitrile) and piperidine were from Sigma and DMF (dimethylformamide) (>99.8%), chloroform, DiD fluorescent dye (3H-Indolium, 2-(5-(1,3-dihydro-3,3-dimethyl-1-octadecyl-2H-indol-2-ylidene)-1,3-pentadienyl)-3,3-dimethyl-1-octadecyl-, perchlorate), Minimum Essential Media was purchased from Thermo Fisher. IgE^dansyl (clone 27–74) were purchased from BD Biosciences. DSPC (1,2-distearoyl-sn-glycero-3-phosphocholine), DSPE-mPEG2000 (1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (ammonium salt)), membranes and all mini extruder components were purchased from Avanti Polar Lipids (Alabaster, Al, USA). DNP-BSA conjugate was purchased from Invitrogen.

Deak P.E., Vrabel M.R., Pizzuti V.J., Kiziltepe T, & Bilgicer B. (2016). Nanoallergens: A multivalent platform for studying and evaluating potency of allergen epitopes in cellular degranulation. Experimental Biology and Medicine, 241(9), 996-1006.

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Quantifying Redox-Sensitive Protein Modifications

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Monobromobimane (MBB), Tris (2-carboxyethyl)phosphine hydrochloride (TCEP), sulfosalicylic acid (SSA), 1-fluoro-2,4-dinitrobenzene (DNFB), TPP® tissue culture dishes, NU6027, penicillin/streptomycin, and t-BOOH were purchased from Sigma (St. Louis, MO). Fetal bovine serum, Dulbecco’s modified Eagle’s medium (DMEM) (standard liquid media) were from Invitrogen (Rockville, MD). β-cyano-L-alanine and diallyl trisulfide were purchased from the Cayman Chemical Company (Ann Arbor, MI). Antibodies used were anti-ATR-pS435 (Cell Signaling Technology Inc, Danvers, MA, catalog number 2853s), anti-total-ATR (Invitrogen, Waltham, MA USA, catalog number PA1–450), anti-CHK1-pS345 (Invitrogen, catalog number PA5–34625), anti-total-CHK1(Abcam, Cambridge, MA, catalog number ab47574), anti-3-MST (Santa Cruz Biotechnology, Santa Cruz, CA, catalog number sc-135993), anti-CSE (Santa Cruz Biotechnology, catalog number sc-101924), anti-CBS (Santa Cruz Biotechnology, catalog number sc-67154), and anti-Visfatin (Nampt, Bethyl Laboratories, Montgomery TX, catalog number A300–779A), rabbit anti-GADPH (Sigma, catalog number G9545), and anti-beta-actin (abcam, catalog number ab8227). Secondary antibodies were goat anti-rabbit IgG (catalog numbers ab6721 & ab2040, Abcam), goat anti-mouse IgG (catalog number ab205719, Abcam), and goat anti-mouse IgG (catalog number sc358920, Santa Cruz).

Chen J., Shen X., Pardue S., Meram A.T., Rajendran S., Ghali G.E., Kevil C.G, & Shackelford R.E. (2018). The Ataxia telangiectasia-mutated and Rad3-related Protein Kinase Regulates Cellular Hydrogen Sulfide Concentrations. DNA repair, 73, 55-63.

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Preparation of DNFB and Nalbuphine Solution

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1-Fluoro-2,4-dinitrobenzene and nalbuphine hydrochloride were purchased from Sigma-Aldrich (St. Louis, MO). DNFB 0.1% solution was prepared in acetone and nalbuphine was dissolved in saline.

Inan S., Huerta A.T., Jensen L.E., Dun N.J, & Cowan A. (2019). Nalbuphine, a kappa opioid receptor agonist and mu opioid receptor antagonist attenuates pruritus, decreases IL-31, and increases IL-10 in mice with contact dermatitis. European journal of pharmacology, 864, 172702.

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Carcinoma Cell Line Culturing

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The HT29 human carcinoma cell line was purchased from Dainihon Pharmacy Co. (Tokyo, Japan). The CT26 murine colon carcinoma cell line was a gift from Professor I. J. Fidler (MD Anderson Cancer Center, TX, USA). Cells were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum at 37°C in 5% CO₂.
1-Fluoro-2,4-dinitrobenzene (DNFB), human recombinant EGF, mouse recombinant EGF, phosphocreatine (pCr), oligomycin (Sigma), ATP, and cyclocreatine (Wako Pure Chemical Corp. Ltd., Osaka, Japan) were purchased from the indicated manufacturers.

Kita M., Fujiwara-Tani R., Kishi S., Mori S., Ohmori H., Nakashima C., Goto K., Sasaki T., Fujii K., Kawahara I., Bhawal U.K., Luo Y, & Kuniyasu H. (2023). Role of creatine shuttle in colorectal cancer cells. Oncotarget, 14, 485-501.

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Synthesis of Opioid Peptide PK20

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PK20 (H–Dmt–D–Lys–Phe–Phe–Lys–Lys–Pro–Phe–Tle–Leu–OH) was synthesized by using solid-phase peptide synthesis according to the method of Merrifield with Fmoc approach [77 (link)] in the Department of Neuropeptides. Chemicals and reagents were purchased from commercial companies: 1-fluoro-2, 4-dinitrobenzene (DNFB, No. 556971), dexamethasone (No. D4902), dinitrobenzene sulfonic acid (DNS, No. D1529), protease inhibitor cocktail (No. S8830), and TX-100 (No. X100) were obtained from Sigma-Aldrich (Poznań, Poland). Ketamine, xylazine, and pentobarbital sodium were purchased from Biowet (Puławy, Poland).

Russjan E., Andrzejewski K., Sulejczak D., Kleczkowska P, & Kaczyńska K. (2019). Endomorphin-2- and Neurotensin- Based Chimeric Peptide Attenuates Airway Inflammation in Mouse Model of Nonallergic Asthma. International Journal of Molecular Sciences, 20(23), 5935.

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Tobramycin Quantification Protocol

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Tobramycin base was supplied by Lisapharma (Erba, CO, Italy). Tris (dydroxymethyl) aminomethane (TRIS) was purchased from Bio-Rad Laboratories (Hercules, CA, USA).
Dimethylsulfoxide (DMSO), 1-Fluoro-2,4-dinitrobenzene, sulfuric acid (≥ 95%), ethanol (100%) and acetonitrile were of analytical/HPLC grade and used as provided by Sigma-Aldrich (Sydney, Australia). High purity water was purified by reverse osmosis (MilliQ, Millipore, France).

Zhu B., Padroni M., Colombo G., Phillips G., Crapper J., Young P.M, & Traini D. (2016). The development of a single-use, capsule-free multi-breath tobramycin dry powder inhaler for the treatment of cystic fibrosis. International journal of pharmaceutics, 514(2).

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