Astra software package version 6

Manufactured by Wyatt Technology

The Astra software package version 6.1 is a product offered by Wyatt Technology. It is a data analysis and reporting tool designed for use with Wyatt's light scattering instrumentation. The software provides functionality for the acquisition, analysis, and presentation of data obtained from Wyatt's light scattering devices.

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Lab products found in correlation

Dawn heleos 2, by Wyatt Technology (2 mentions) Superdex 200, by Wyatt Technology (1 mentions) Superose 6 column, by Wyatt Technology (1 mentions) Superdex 200 10 300 gl, by Wyatt Technology (1 mentions) Superdex 200 10 300 gl column, by GE Healthcare (1 mentions) Dawn heleos 2 mals detector, by Wyatt Technology (1 mentions)

Close spelling variants of this product

ASTRA 6 software (413 citations) ASTRA software (357 citations) ASTRA software package (52 citations) ASTRA VI software (30 citations) ASTRA software version 6.1 (21 citations) ASTRA Version 6.1.6.5 (20 citations)

4 protocols using astra software package version 6

Multi-Angle Light Scattering Analysis

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Standard multi-angle light scattering experiments were carried out with a Superdex 200 (10/300 GL) connected in-line to a Dawn Heleos II multi-angle light-scattering (MALS) detector (Wyatt Technologies, Santa Barbara, CA). 100 µL samples (2 mg/mL or 4 mg/mL) were injected at a flow rate of 0.3 mL/min in a column equilibrated in 10 mM HEPES, pH 7.5, 150 mM NaCl, and 2 mM tris(2-carboxyethyl)phosphine (TCEP) buffer. Molecular weights and standard deviations were determined using Astra software package version 6.1 (Wyatt Technology Corporation, Santa Barbara, CA). All experiments were performed at room temperature and at least in duplicate.

Johnson B., Li J., Adhikari J., Edwards M.R., Zhang H., Schwarz T., Leung D.W., Basler C.F., Gross M.L, & Amarasinghe G.K. (2016). Dimerization controls Marburg virus VP24-dependent modulation of host antioxidative stress responses. Journal of molecular biology, 428(17), 3483-3494.

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Determining Protein Mass and Oligomerization

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The molecular mass and the oligomerization state of the purified MsGabP was determined via size exclusion chromatography coupled to light scattering, absorbance, and differential refractive index detectors method. The refractive index and light scattering detectors were from Wyatt Technology (Goleta, CA) and the UV detector and chromatography pumps from Shimadzu Corporation (Kyoto, Japan). The Superose 6 column (WTC-MP030S5; Wyatt Technology, Goleta, CA) was equilibrated with 20 mM HEPES (pH 7.0), 100 mM NaCl, 5% (vol/vol) glycerol, 0.0102% (wt/vol) DMNPG overnight. Thirty microliters of MsGabP (3 mg/ml) was injected and the sample eluted from the column was analyzed by three detectors (30 (link)). Data obtained were analyzed using ASTRA software package, version 6.1 (Wyatt Technology, Goleta, CA). The program calculated M_W,protein, M_W,detergent, and M_W,total throughout the peak and also provided information on the monodispersity of the peak (56 (link)).

Pavić A., Ji Y., Serafini A., Garza-Garcia A., McPhillie M.J., Holmes A.O., de Carvalho L.P., Wang Y., Bartlam M., Goldman A, & Postis V.L. (2021). Functional Characterization of the γ-Aminobutyric Acid Transporter from Mycobacterium smegmatis MC2 155 Reveals Sodium-Driven GABA Transport. Journal of Bacteriology, 203(4), e00642-20.

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Size Exclusion Chromatography with MALS

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All experiments were carried out with a Superdex 200 (10/300 GL) connected in-line to a Dawn Heleos II multi-angle light-scattering (MALS) detector (Wyatt Technologies, Santa Barbara, CA). For each experiment, 100 μL of proteins at 2 mg/mL were injected into the column equilibrated in 20 mM Tris, pH 7.5, 150 mM NaCl, and 2 mM tris(2-carboxyethyl)phosphine (TCEP) buffer at room temperature. Molecular weights were determined using Astra software package version 6.1 (Wyatt Technology Corporation, Santa Barbara, CA). All experiments were performed at least in duplicate.

Su Z., Wu C., Shi L., Luthra P., Pintilie G.D., Johnson B., Porter J.R., Ge P., Chen M., Liu G., Frederick T.E., Binning J.M., Bowman G.R., Zhou Z.H., Basler C.F., Gross M.L., Leung D.W., Chiu W, & Amarasinghe G.K. (2018). Electron cryo-microscopy structure of Ebola nucleoprotein reveals a mechanism for nucleocapsid-like assembly. Cell, 172(5), 966-978.e12.

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SEC-MALS Analysis of Protein

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Standard MALS experiments were carried out on a Superdex 200 10/300 GL column (GE Healthcare) connected in-line to a Dawn Heleos II MALS detector (Wyatt Technologies). 100 μL samples (2 mg/mL) were injected at a flow rate of 0.3 mL/minute into a column equilibrated in 10 mM HEPES pH 7.5, 150 mM NaCl, and 2 mM tris(2-carboxyethyl)phosphine (TCEP) buffer. Molecular weights and standard deviations were determined using Astra software package version 6.1 (Wyatt Technologies). All experiments were performed at room temperature and in triplicate.

Hung P.J., Johnson B., Chen B.R., Byrum A.K., Bredemeyer A.L., Yewdell W.T., Johnson T.E., Lee B.J., Deivasigamani S., Hindi I., Amatya P., Gross M.L., Paull T.T., Pisapia D.J., Chaudhuri J., Petrini J.J., Mosammaparast N., Amarasinghe G.K., Zha S., Tyler J.K, & Sleckman B.P. (2018). MRI is a DNA Damage Response Adaptor during Classical Non-Homologous End Joining. Molecular cell, 71(2), 332-342.e8.

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