Single cell suspensions of collagenase-digested tumors were stained with the following antibodies purchased from eBioscience: fixable viability dye efluor 450, CD69-FITC, PD-1-PE, CD4-PeCy7, CD45-Alexa Fluor 700, CD8a-PE efluor 610, CD40-FITC, CD70-PE, MHC-II IAd-APC, CD11c-PE efluor 610, CD45-APC, CD3-PerCP-Cy5.5, CD11b-PerCP-Cy5.5, EpCAM-PeCy7, PD-L1-PE and PD-L2-FITC. Phospho-Smad2/3 levels were assessed as previously described (27 (link)). Briefly, TDLN cells were stained with anti-mouse CD4-PE and anti-mouse CD8-FITC (eBioscience), fixed, permeabilized (Foxp3 Fixation/Permeabilization Concentrate and Diluent kit, eBioscience) and stained with goat anti-phospho-Smad2/3 (Ser 423/425) followed by APC-labeled donkey anti-goat IgG (Santa Cruz Biotechnology). All samples were acquired with LSRII flow cytometer and analyzed with FlowJo software (version 7.3.6).
Pd l2 fitc
Manufactured by Thermo Fisher Scientific
PD-L2-FITC is a fluorescently labeled antibody used for the detection and analysis of PD-L2 (Programmed Death-Ligand 2) expression on cells. It is a useful tool for flow cytometry and other immunoassays.
Automatically generated - may contain errors
Lab products found in correlation
Pd 1 pe, by Thermo Fisher Scientific (2 mentions)
Cd40 fitc, by Thermo Fisher Scientific (1 mentions)
Cd11b percp cy5, by Thermo Fisher Scientific (1 mentions)
Cd69 fitc, by Thermo Fisher Scientific (1 mentions)
Cd4 pe cy7, by Thermo Fisher Scientific (1 mentions)
Cd45 alexa fluor 700, by Thermo Fisher Scientific (1 mentions)
Cd45 apc, by Thermo Fisher Scientific (1 mentions)
Cd3 percp cy5, by Thermo Fisher Scientific (1 mentions)
Epcam pe cy7, by Thermo Fisher Scientific (1 mentions)
Pd l1 pe, by Thermo Fisher Scientific (1 mentions)
Anti mouse cd4 pe, by Thermo Fisher Scientific (1 mentions)
Anti mouse cd8 fitc, by Thermo Fisher Scientific (1 mentions)
Foxp3 fixation permeabilization concentrate and diluent kit, by Thermo Fisher Scientific (1 mentions)
Lsrii flow cytometer, by FlowJo (1 mentions)
Efluor 450, by Thermo Fisher Scientific (1 mentions)
Cd206, by Santa Cruz Biotechnology (1 mentions)
Cd25 apc, by Thermo Fisher Scientific (1 mentions)
Cd226 pe, by BioLegend (1 mentions)
Cellquest software, by BD (1 mentions)
Pd l1 pe cy7, by Thermo Fisher Scientific (1 mentions)
2 protocols using pd l2 fitc
1
Multiparameter Tumor Immune Profiling
2
Multiparameter Flow Cytometry of Immune Cells
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Single cell suspensions were stained with monoclonal antibodies for 1 hour at 4°C. One to four‐color flow cytometry was performed (FACS LSR, Becton Dickinson). Data were acquired using the CellQuest software (Becton Dickinson) using at least 100 000 events gated for live cells.
The following conjugated rat anti‐mouse mAbs were used: CD45‐PerCP, CD11b‐APC, CCR4‐PE, CCR5‐PE, CCR7‐PE, CCR10‐PE and CXCR4‐PE (BD Biosciences); CD49b‐APC (Biolegend); CD226‐PE, CD4‐FITC, CD25‐APC, CD8a‐FITC, PD1‐PE, CTLA4‐PE, PDL1‐PE‐Cy7, PDL2‐FITC, CD47‐APC (eBiosciences), CD11c‐FITC, GR1‐FITC (Miltenyi Biotech), CD206 (Santa Cruz), and anti‐mouse Foxp3 staining set PE (eBioscience).
The following conjugated rat anti‐mouse mAbs were used: CD45‐PerCP, CD11b‐APC, CCR4‐PE, CCR5‐PE, CCR7‐PE, CCR10‐PE and CXCR4‐PE (BD Biosciences); CD49b‐APC (Biolegend); CD226‐PE, CD4‐FITC, CD25‐APC, CD8a‐FITC, PD1‐PE, CTLA4‐PE, PDL1‐PE‐Cy7, PDL2‐FITC, CD47‐APC (eBiosciences), CD11c‐FITC, GR1‐FITC (Miltenyi Biotech), CD206 (Santa Cruz), and anti‐mouse Foxp3 staining set PE (eBioscience).
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