The following compounds were purchased from FUJIFILM Wako Pure Chemical Co. (Osaka, Japan): [6]-gingerol (Lot. APQ6161), amygdalin (Lot. KPM0958), glycyrrhizin (Lot. CAH4202), berberine (Lot. PTM0089), and (Z)-ligustilide (Lot. ESJ5922). The solvents used for HPLC and TLC analyses were of research grade. The TLC plate (Silica Gel 60 F254) was purchased from Merck Co. (Darmstadt, Germany).
Berberine
Manufactured by Fujifilm
Sourced in Japan
Berberine is a naturally occurring compound that can be used in various laboratory applications. It is a yellow crystalline alkaloid found in several plant species. Berberine has been studied for its potential biological properties, but its core function is to serve as a research tool in scientific investigations.
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2 protocols using berberine
1
Analytical Characterization of Bioactive Compounds
2
Kampo Herbal Extract Preparation and Use
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Kampo medicines are aqueous extracts of a mixture of natural crude drugs. Eight kinds of Kampo extracts, HST, OGT, HJG (hachimi-jio-gan), SST (sho-saiko-to), HET (hochu-ekki-to), JTT (juzen-taiho-to), DKT (dai-kenchu-to), and SRT (sai-rei-to) were obtained from Tsumura and Co. (Tokyo, Japan) in the form of dried powdered extract. Berberine and wogonin were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Alternatively, wogonin was extracted from Scutellaria root at Tsumura and Co. at a purity of more than 99.5% for the in vivo studies.
The Kampo extracts were suspended in dimethyl sulfoxide (DMSO) at 10 mg/mL and passed through a 0.22 μm membrane following gentle centrifugation. The supernatant fluids were added to the cultures at a final concentration of 10 μg/mL. The wogonin and Berberine were dissolved in DMSO and added to the cultures at the described concentrations.
The Kampo extracts were suspended in dimethyl sulfoxide (DMSO) at 10 mg/mL and passed through a 0.22 μm membrane following gentle centrifugation. The supernatant fluids were added to the cultures at a final concentration of 10 μg/mL. The wogonin and Berberine were dissolved in DMSO and added to the cultures at the described concentrations.
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