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Mouse gadd34

Manufactured by Thermo Fisher Scientific
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Mouse GADD34 is a laboratory product that serves as a regulator of cellular stress response. It plays a role in the regulation of protein synthesis and cell growth. Further details on its specific applications or intended use are not available.

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Lab products found in correlation

Human 18s rrna, by Thermo Fisher Scientific (2 mentions) Human chop, by Thermo Fisher Scientific (2 mentions) Human gadd34, by Thermo Fisher Scientific (2 mentions) Mouse chop, by Thermo Fisher Scientific (2 mentions) Mouse β actin, by Thermo Fisher Scientific (2 mentions) Rat β actin, by Thermo Fisher Scientific (2 mentions) Superscript 4 reverse transcriptase, by Thermo Fisher Scientific (2 mentions)

2 protocols using mouse gadd34

1

Quantitative RT-PCR of ER Stress Genes

Check if the same lab product or an alternative is used in the 5 most similar protocols
300 ng of total RNA was reverse transcribed using SuperScript IV Reverse Transcriptase (Thermo Scientific). The resulting cDNA was then used for real-time PCR (15 ng per reaction) with the following TaqMan assays: human CHOP, human GADD34, human 18S rRNA, mouse CHOP, mouse GADD34, mouse β-actin, rat β-actin, and GFP (all Thermo Scientific). The resulting Ct values were analyzed using the 2ΔΔCt method and expressed as fold change.
Nelson A.T., Cicardi M.E., Markandaiah S.S., Han J.Y., Philp N.J., Welebob E., Haeusler A.R., Pasinelli P., Manfredi G., Kawamata H, & Trotti D. (2024). Glucose hypometabolism prompts RAN translation and exacerbates C9orf72-related ALS/FTD phenotypes. EMBO Reports, 25(5), 2479-2510.
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2

Quantifying ER Stress Response Genes

Check if the same lab product or an alternative is used in the 5 most similar protocols
300 ng of total RNA was reverse transcribed using SuperScript IV Reverse Transcriptase (Thermo Scientific). The resulting cDNA was then used for real-time PCR (15 ng per reaction) with the following TaqMan assays: human CHOP, human GADD34, human 18S rRNA, mouse CHOP, mouse GADD34, mouse β-actin, rat β-actin, and GFP (all Thermo Scientific). The resulting Ct values were analyzed using the 2ΔΔCt method and expressed as fold change.
Nelson A.T., Cicardi M.E., Markandaiah S.S., Han J., Philp N., Welebob E., Haeusler A.R., Pasinelli P., Manfredi G., Kawamata H, & Trotti D. (2023). Glucose Hypometabolism Prompts RAN Translation and Exacerbates C9orf72-related ALS/FTD Phenotypes. bioRxiv.
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