Bone marrow cells from female C57BL/6 mice were removed aseptically from femurs and tibias by flushing with ice-cold PBS. After red blood cell lysis, T cells were depleted from bone marrow cells by incubation with anti-Thy 1.2 antibody (BioLegend) for 30 min at 4°C, followed by another incubation with Low-TOX-M rabbit complement (Cedarlane) for 40 min at 37°C. Splenic T cells were purified by positive selection using anti-CD5 antibody-conjugated magnetic beads (Miltenyi, Auburn, CA). TCD-BM cells (5 × 106) were washed and resuspended in 100 μL PBS and injected into lethally irradiated recipient female B6D2F1 mice through the lateral tail vein. To induce GVHD, 1 × 106 splenic T cells were injected together with 5 × 106 TCD-BM cells into recipient mice. B6D2F1 mice received twice total body irradiation (5.5 Gy each) with a 3 h interval between each irradiation to minimize the gastrointestinal toxicity.
Anti cd5 antibody conjugated magnetic beads
Manufactured by Miltenyi Biotec
Anti-CD5 antibody-conjugated magnetic beads are a laboratory product that can be used to isolate and enrich CD5-positive cells from biological samples. The beads are coated with antibodies specific to the CD5 antigen, allowing for the selective capture and separation of CD5-expressing cells through magnetic separation techniques. This product is designed for research applications and does not include any interpretations or extrapolations about its intended use.
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Lab products found in correlation
2 protocols using anti cd5 antibody conjugated magnetic beads
1
Graft-versus-Host Disease Mouse Model
2
Allogenic Hematopoietic Stem Cell Transplantation
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BM cells from C57BL/6 mice were removed aseptically from femurs and tibias, and T cells were depleted by incubation with anti-Thy 1.2 antibody for 30 min at 4°C, followed by incubation with Low-TOX-M rabbit complement (Cedarlane) for 40 min at 37°C. Splenic T cells were purified by positive selection using anti-CD5 antibody-conjugated magnetic beads (Miltenyi, Auburn, CA). TCD-BM cells of 5 × 106 with or without 1 × 106 splenic T cells (to induce GVHD) were washed and resuspended in PBS and injected into lethally irradiated recipient B6D2F1 mice through the lateral tail vein. B6D2F1 mice received a total of 11-Gy total-body irradiation with a split of 3 h interval to minimize the gastrointestinal toxicity.
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