Fei tecnai t12 microscope

SERS measurements were conducted using a home-built, inverted high-speed Raman confocal microscope (Figure S5) equipped with a compact LM series volume holographic grating-stabilized 785 nm near-infrared diode laser, a 60× oil immersion objective lens with a numerical aperture of 0.65–1.25 (RMS60X-PFOD, Olympus), a HoloSpec f/1.8 spectrograph and an iDus CCD Camera. The detailed information related to the set-up can be found in our previous work.^{24 (link)–27} The SERS measurements were acquired at room temperature with 100 ms integration time and a laser power of 5 mW at the sample, unless otherwise mentioned.
UV-vis extinction spectra were collected on an Aviv Model 14DS UV-vis spectrophotometer (Aviv Biomedical, Lakewood, NJ). qPCR was performed on a CFX96 Touch real-time PCR detection system (Bio-Rad, Hercules, CA). Transmission electron microscopic (TEM) images were taken with an FEI Tecnai T12 microscope (FEI, Hillsboro, OR) at an accelerating voltage of 120 kV. The air-dried samples were prepared through deposition of a drop of samples onto the TEM 200-mesh copper grid (Ted Pella, Redding, CA).

Room temperature TEM images of IONPs were acquired with a FEI Tecnai T12 microscope (FEI, Inc., Hillsboro, OR) operating at 120 kV. A 200 mesh copper grid with formvar and carbon supports was dipped into a ~1 mg Fe/ml IONP suspension, then removed and allowed to dry before imaging. The LNCaP cells underwent a standard process of fixation, staining, dehydration, infiltration of polymer resin, and curing. The final polymer block was cut into ~60 nm slices with a microtome, deposited on a 200 mesh copper grid with formvar and carbon supports (Ted Pella Inc., Redding, CA), and imaged at 60 kV. Multiple images were taken at high magnification and later combined in a collage to obtain the high resolution image of the whole cell presented in Fig. 4. Cryogenic TEM imaging was performed on a FEI Tecnai Spirit Bio-Twin microscope (FEI, Inc. Hillsboro, OR) at 120 kV and −179 °C.

Grids were prepared by applying 3 μL of purified complex at a concentration of 25 μg/mL to a glow-discharged 400 mesh copper grid covered by a thin layer of continuous formvar carbon film (EMS) and stained with 2% uranium formate. The grids were imaged on a FEI Tecnai T12 microscope (Thermo Fisher Scientific) operated at 120 kV at a nominal magnification of 52,000x using an UltraScan 400 camera (Gatan), corresponding to a pixel size of 2.21 Å on the specimen.