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Anti cd3 fitc antibody

Manufactured by BioLegend
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The Anti-CD3-FITC antibody is a laboratory reagent used for the detection and identification of CD3-positive cells. CD3 is a protein complex that is expressed on the surface of T cells and plays a crucial role in T cell activation and signal transduction. The Anti-CD3-FITC antibody is conjugated with the fluorescent dye FITC (Fluorescein Isothiocyanate), which allows for the visualization and quantification of CD3-positive cells using flow cytometry or fluorescence microscopy techniques.

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Lab products found in correlation

Anti cd45 percp antibody, by BioLegend (3 mentions) Anti cd137 apc antibody, by BioLegend (3 mentions) Anti cd137 mab, by BPS Biosciences (3 mentions) Cyto x cytoperm, by BD (2 mentions) Cytofix cytoperm, by BD (1 mentions) Anti cd11b apc, by BD (1 mentions) Anti cd45 fitc, by BD (1 mentions) Anti gr 1 percp cy5, by BD (1 mentions) Anti cd31, by Abcam (1 mentions) Nf kb p65 f 6, by Santa Cruz Biotechnology (1 mentions) Pe anti f4 80, by BD (1 mentions)

4 protocols using anti cd3 fitc antibody

1

Flow Cytometric Analysis of CD137, CD8+ T Cell Proliferation, and NF-κB in Gastric Cancer

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For CD137 detection, the PBMCs or TILs of GC patients were placed in flow tubes, and 5 µL each of an anti-CD45-PerCP antibody (BioLegend, USA), anti-CD3-FITC antibody (BioLegend, USA) and anti-CD137-APC antibody (BioLegend, USA) was added. The cells were incubated in the dark for 10 min and washed with PBS once. PBS (200 µL) was added for flow cytometry detection.
For examination of CD8+ T cells proliferation, CD8+ T cells of GC patients were placed in flow tubes and washed once with PBS. PBS (200 µL) was added for flow cytometry detection.
For NF-κB detection, CD8+ T cells from GC patients were treated with 10 µg/ml anti-CD137 mAb (BPS Bioscience, USA) for 72 h and placed in flow tubes. After washing once with PBS, a Fixation/Permeabilization Solution (BD Cytofix/Cytoperm™) was added at room temperature for 30 min. After washing once with PBS, an NF-κB p65 rabbit mAb (1:1000, CST, USA) was added. The cells were incubated in the dark for 1 h and washed once with PBS. Five microliters of anti-rabbit IgG (H+L), F(ab’)2 Fragment (Alexa Fluor® 594 Conjugate) (1:500, CST, USA) was added, and the cells were incubated in the dark for 30 min. After washing once with PBS, 200 µL of PBS was added for flow cytometry detection.
Hu B.S., Tang T., Jia J.L., Xie B.C., Wu T.L., Sheng Y.Y., Xue Y.Z, & Tang H.M. (2020). CD137 agonist induces gastric cancer cell apoptosis by enhancing the functions of CD8+ T cells via NF-κB signaling. Cancer Cell International, 20, 513.
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2

Mouse Model for Liver Inflammation

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Eighty male C3H mice (6 weeks old, weighing 20–25 g) were purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd. (Beijing, china), and kept in a specific pathogen free mouse breeding room with controlled temperature of 25 ± 1°C. The mice were provided free access to food.
Anti-CD45 FITC, anti-F4/80 PE, anti-CD11b APC, and anti-Gr1PerCP-Cy5.5 were purchased from BD Biosciences (Lake Franklin, New Jersey, United States). Anti-CD3 FITC antibody was purchased from BioLegend (San Diego, California, United States). Primary antibodies against WNT1 (H-89) and NF-KB P65(F-6) were purchased from Santa Cruz Biotechnology Co., Ltd. (Santa Cruz Avenue, California, United States). Primary antibodies against VEGFA (VG-1), β-catenin, and APC were purchased from Abcam (Cambridge, MA, United States). Primary antibody against β-actin was purchased from Huaan Biotechnology Co., Ltd. (Hangzhou, China). Anti-CD31 was purchased from Abcam. DEN was purchased from Merck Group (Darmstadt, Germany).
Cheng C., Shou Q., Lang J., Jin L., Liu X., Tang D., Yang Z, & Fu H. (2020). Gehua Jiecheng Decoction Inhibits Diethylnitrosamine-Induced Hepatocellular Carcinoma in Mice by Improving Tumor Immunosuppression Microenvironment. Frontiers in Pharmacology, 11, 809.
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3

Evaluating CD137 and NF-κB in GC Immunology

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For CD137 detection, the PBMCs or TILs of GC patients were placed in ow tubes, and 5 µL each of an anti-CD45-PerCP antibody (BioLegend, USA), anti-CD3-FITC antibody (BioLegend, USA) and anti-CD137-APC antibody (BioLegend, USA) was added. The cells were incubated in the dark for 10 min and washed with PBS once. PBS (200 µL) was added for ow cytometry detection.
For examination of CD8 + T cells proliferation, CD8 + T cells of GC patients were placed in ow tubes and washed once with PBS. PBS (200 µL) was added for ow cytometry detection.
For NF-κB detection, CD8 + T cells from GC patients were treated with 10 µg/ml anti-CD137 mAb (BPS Bioscience, USA) for 72 h and placed in ow tubes. After washing once with PBS, a Fixation/Permeabilization Solution (BD Cyto x/Cytoperm TM ) was added at room temperature for 30 min. After washing once with PBS, an NF-κB p65 rabbit mAb (1:1000, CST, USA) was added. The cells were incubated in the dark for 1 h and washed once with PBS. Five microliters of anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 594 Conjugate) (1:500, CST, USA) was added, and the cells were incubated in the dark for 30 min. After washing once with PBS, 200 µL of PBS was added for ow cytometry detection.
Hu B., Tang T., Jia J., Xie B., Wu T., Sheng Y., Xue Y., & Tang H. (2020). CD137 agonist induces gastric cancer cell apoptosis by enhancing the functions of CD8+ T cells via NF-κB signaling.
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4

Evaluating CD137 and NF-κB in GC Immunology

Check if the same lab product or an alternative is used in the 5 most similar protocols
For CD137 detection, the PBMCs or TILs of GC patients were placed in ow tubes, and 5 µL each of an anti-CD45-PerCP antibody (BioLegend, USA), anti-CD3-FITC antibody (BioLegend, USA) and anti-CD137-APC antibody (BioLegend, USA) was added. The cells were incubated in the dark for 10 min and washed with PBS once. PBS (200 µL) was added for ow cytometry detection.
For examination of CD8 + T cells proliferation, CD8 + T cells of GC patients were placed in ow tubes and washed once with PBS. PBS (200 µL) was added for ow cytometry detection.
For NF-κB detection, CD8 + T cells from GC patients were treated with 10 µg/ml anti-CD137 mAb (BPS Bioscience, USA) for 72 h and placed in ow tubes. After washing once with PBS, a Fixation/Permeabilization Solution (BD Cyto x/Cytoperm TM ) was added at room temperature for 30 min. After washing once with PBS, an NF-κB p65 rabbit mAb (1:1000, CST, USA) was added. The cells were incubated in the dark for 1 h and washed once with PBS. Five microliters of anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 594 Conjugate) (1:500, CST, USA) was added, and the cells were incubated in the dark for 30 min. After washing once with PBS, 200 µL of PBS was added for ow cytometry detection.
Hu B., Tang T., Jia J., Xie B., Wu T., Sheng Y., Xue Y., & Tang H. (2020). CD137 agonist induces gastric cancer cell apoptosis by enhancing the functions of CD8+ T cells via NF-κB signaling.
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