From 2 mg of lipids, MLVs were obtained with ratio 1:50 (compound/lipids). The samples were placed in DSC Tzero hermetic pans and the scanning was carried out over a 10–40 °C temperature at a heating rate of 1 °C/min in DSC Q25 equipment (TA Instruments, USA); HEPES buffer was used as reference solution. To obtain the temperature of phase transition (Tm) and the transition enthalpy (DH), the Trios software (TA Instruments) was used in the analysis of the thermograms.
Dsc q25
Manufactured by TA Instruments
Sourced in United States
The DSC Q25 is a Differential Scanning Calorimeter (DSC) manufactured by TA Instruments. It is a thermal analysis instrument used to measure the heat flow associated with phase transitions and chemical reactions in materials as a function of temperature and time.
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6 protocols using dsc q25
1
Liposomal Phase Transition Analysis
2
Thermodynamic Analysis of Peptide-Lipid Interactions
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DSC analysis was made using a DSC Q25 (TA instrument, New Castle, DE, USA). MLVs were prepared using 1 mg of lipids to give two peptide–lipid ratios: 1:50 and 1:10. HEPES buffer was used as a reference solution. Samples were encapsulated in standard aluminum DSC pans, and scanning was carried out over a range of 10 °C–40 °C at a heating rate of 1 °C/min. Thermograms were acquired and analyzed using the Trios software package (TA Instruments) to obtain the phase transition temperature (Tm), the transition enthalpy (DH), and the FWHM.
3
Differential Scanning Calorimetry and Thermogravimetric Analysis of Cellulose Materials
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Differential scanning calorimetry
(DSC) data were obtained on a TA Instruments DSC Q25. DSC experiments
were performed on 3.0–5.0 mg samples in hermetically sealed
aluminum pans (TA Instruments). Samples of CM and CM-dye were heated
at 5.0 °C/min over the temperature range 25–130 °C.
Values cited refer to triplicate measurements.
Thermogravimetric
(TGA) data were obtained on a TA Instruments SDT Q600 or Q50 (New
Castle, DE) using a nitrogen flow rate of 50 mL/min. All experiments
used ∼3.0 mg of CM or CM-dye in open 90 μL aluminum pans
(TA Instruments). Isothermal experiments were performed at 50, 55,
60, and 65 °C. The fraction dehydrated, α, at any given
time was determined from the wt % loss at each data point relative
to the total wt % loss (CM calc. = 13.9 wt %). The α values
obtained were used in model-based and model-free kinetic analyses.
(DSC) data were obtained on a TA Instruments DSC Q25. DSC experiments
were performed on 3.0–5.0 mg samples in hermetically sealed
aluminum pans (TA Instruments). Samples of CM and CM-dye were heated
at 5.0 °C/min over the temperature range 25–130 °C.
Values cited refer to triplicate measurements.
Thermogravimetric
(TGA) data were obtained on a TA Instruments SDT Q600 or Q50 (New
Castle, DE) using a nitrogen flow rate of 50 mL/min. All experiments
used ∼3.0 mg of CM or CM-dye in open 90 μL aluminum pans
(TA Instruments). Isothermal experiments were performed at 50, 55,
60, and 65 °C. The fraction dehydrated, α, at any given
time was determined from the wt % loss at each data point relative
to the total wt % loss (CM calc. = 13.9 wt %). The α values
obtained were used in model-based and model-free kinetic analyses.
4
Thermogram Analysis of Multillamellar Vesicles
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For the acquisition of thermograms by DSC analysis, a TA instrument DSC Q25 was used. Multillamellar vesicles (MLVs) were prepared using 2 mg of lipids hydrated with Bz-Im diluted in HEPES buffer to give three Bz-Im–lipid ratios: 1:50, 1:25, and 1:10. HEPES buffer was used as a reference solution. The samples were placed and subsequently sealed within standard aluminum DSC pans, and were analyzed over a range of 10 to 35 °C at a heating rate of 1 °C/min. Trios software (TA Instruments) was used to obtain the phase transition temperature (Tm), the transition enthalpy (ΔH), and the full width at half-maximum from thermograms (FWHM, ΔTm/2).
5
Differential Scanning Calorimetry of PEO Electrolytes
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The differential
scanning calorimetry (DSC) samples were prepared by putting ∼8–10
mg of material in aluminum pans provided by TA Instruments. DSC experiments
of the neat PEOs and their electrolytes were carried out with a TA
Instruments DSC Q25 instrument equipped with a refrigerated cooling
system. An aluminum pan was used as a reference. To eliminate the
temperature history completely, all of the samples were heated to
120 °C and waited for 5 min. Samples were then quickly cooled
down to −85 °C at the rate of 20 °C/min. DSC scans
were then collected at the rate of 10 °C/min during the heating
process to 120 °C. Eventually, the step of the heat capacity
in DSC measurements was used to estimate the glass-transition temperatures
for the samples.
scanning calorimetry (DSC) samples were prepared by putting ∼8–10
mg of material in aluminum pans provided by TA Instruments. DSC experiments
of the neat PEOs and their electrolytes were carried out with a TA
Instruments DSC Q25 instrument equipped with a refrigerated cooling
system. An aluminum pan was used as a reference. To eliminate the
temperature history completely, all of the samples were heated to
120 °C and waited for 5 min. Samples were then quickly cooled
down to −85 °C at the rate of 20 °C/min. DSC scans
were then collected at the rate of 10 °C/min during the heating
process to 120 °C. Eventually, the step of the heat capacity
in DSC measurements was used to estimate the glass-transition temperatures
for the samples.
6
Cocrystal Screening and Characterization of Ila/Xyl
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For cocrystal screening of Ila and characterization analysis of Ila/Xyl cocrystals using DSC, a TA Instruments DSC Q25 (TA Instruments, Philadelphia, PA, USA). Additionally, samples were placed in an aluminum pan, ranging from 4.5 mg to 5 mg, and sealed using a lid. The sampling process utilized Tzero pan and lid (TA Instruments, Philadelphia, PA, USA). The DCS instrument was calibrated using indium as standards with regard to temperature and enthalpy. The thermal profile was analyzed in a nitrogen atmosphere (nitrogen flow: 50 mL/min) from 30 °C to 350 °C at a scan rate of 10 °C/min.
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