Imaging was performed on an Applied Precision DeltaVision (GE healthcare) deconvolution microscope. The phagocytosis assay was prepared as stated above with 3×105 macrophages per well in an 8 well Lab-Tek Chambered Coverglass (Thermo Scientific). 1×105 CFSE labeled B16F10 cells were incubated with the proteins and then added to the macrophages for at least 3 hours before imaging. Samples were stained with anti-human CD14 Alexa Fluor 647 antibody before imaging on the DeltaVision microscope. Images were recorded on the 20× lens and 60× oil lens.
Applied precision deltavision
Manufactured by GE Healthcare
Sourced in United States
The Applied Precision DeltaVision is a high-performance, wide-field fluorescence microscope system designed for live-cell imaging and three-dimensional (3D) reconstruction of biological samples. It utilizes a unique optical design to provide high-resolution imaging capabilities.
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Lab products found in correlation
2 protocols using applied precision deltavision
1
Phagocytosis of Labeled Tumor Cells
2
Mitochondrial Membrane Potential in Fibroblasts
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The mitochondrial membrane potential was assessed in live primary fibroblasts using 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine iodide (JC-1; Sigma-Aldrich) as described [26 (link)]. Briefly, fibroblasts were washed and incubated with 5 µg/ml JC-1 dye for 20 min at 37℃. The cells were then rinsed with culture medium, and their images were obtained using the Applied Precision DeltaVision fluorescence microscopy system (GE Healthcare, Chicago, IL, USA). JC-1 accumulates as red fluorescent aggregates within polarized mitochondria but does as green fluorescent monomers within less polarized mitochondria.
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