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2 protocols using anti h4r3me2a antibodies

1

Antibody Characterization for Epigenetic Proteins

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Anti-PRMT1 (F339), Acetyl-Histone H3 (Lys27) (D5E4), anti-CyclinD and anti-β-catenin antibodies were from Cell Signaling. Anti-Lamin B (C20), anti-β-actin were from Santa Cruz. Rabbit Anti-PRMT1 antibody (against aa 300-361), were from Abcam. Anti-asymmetric-dimethyl-arginine antibodies and Anti-H4R3me2a antibodies were from ActiveMotif. Monoclonal anti-HNF4α (H1415) antibodies were from R&D Systems Inc. Mouse anti- β-actin, mouse Monoclonal Anti-PRMT1 clone 171 (against aa 1-361), Anti-JMJD6 (SAB3500233) antibodies were from Sigma-Aldrich, Saint Louis, MO. Anti-GAPDH was from Ambion.
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2

Antibody Profiling for PRMT1 Pathway

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Anti-PRMT1 (F339), anti-pSer MAPK/CDK substrate, anti-H4, anti-Foxo1, anti-FOXO3 (N-terminal), anti-SOD2 and anti-cleaved Caspase3 antibodies were from Cell Signaling. Anti-β-actin, anti-SOD1, anti-p300 antibodies were from Santa Cruz. Rabbit Anti-PRMT1 antibody (against aa 300–361), pan anti-pSer, anti-SAM68 antibodies, were from Abcam. Anti-asymmetric-dimethyl-arginine antibodies, and Anti-H4R3me2a antibodies were from ActiveMotif. Mouse anti- β-actin, mouse Monoclonal Anti-PRMT1 clone 171 (against aa 1–361), Anti-Flag antibodies were from Sigma-Aldrich, Saint Louis, MO.
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