PDLSCs were detached by trypsinization. The cells were incubated with fluorescein isothiocyanate (FITC)-labelled antigen-specific primary antibody or FITC-conjugated control IgG (Bethyl Laboratories, Montgomery, TX, USA) for 30 min on ice. FITC-conjugated anti-CD44 antibody (eBioscience, San Diego, CA, USA), FITC-conjugated anti-CD105 antibody (Millipore, Bedford, MA, USA), FITC-conjugated anti-CD90 antibody (eBioscience), FITC conjugated anti-CD45 antibody (eBioscience), FITC-conjugated anti-CD31 antibody (eBioscience) and FITC-conjugated anti-CD144 antibody (eBioscience) were used as primary antibody. After washing, the cells were suspended in 1% FBS/PBS and analyzed using a Guava easyCyte Flow Cytometer and the InCyte 3.1 software (Merck Millipore, Bedford, MA, USA).
Fitc conjugated anti cd44 antibody
Manufactured by Thermo Fisher Scientific
Sourced in United States
The FITC-conjugated anti-CD44 antibody is a laboratory reagent used for the detection and analysis of CD44 expression in cells. CD44 is a cell surface glycoprotein involved in cell-cell interactions, cell adhesion, and migration. The FITC (fluorescein isothiocyanate) fluorescent label allows for the visualization and quantification of CD44-positive cells using flow cytometry or fluorescence microscopy.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using fitc conjugated anti cd44 antibody
1
Characterization of PDLSCs by Flow Cytometry
2
Isolation of ZIKV-infected Müller Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Müller cells were isolated using the MACS cell separation system (Miltenyi Biotec, San Diego, CA, USA). ZIKVFSS-infected retina tissues were harvested at 6 days post infection (dpi) and digested with collagenase. The cell suspension was first labeled with FITC-conjugated anti-CD44 antibody (eBioscience, San Diego, CA, USA), followed by binding to magnetic bead-conjugated anti-FITC antibody. Tagged cells were isolated with a MidiMACS column (Miltenyi Biotec, San Diego, CA, USA). Both Müller cells and flow-through fractions were collected for downstream RT-PCR analyses.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!