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Eclipse 80i

Manufactured by Canon
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The Eclipse 80i is a high-performance microscope designed for a variety of laboratory applications. It features a durable and ergonomic design, providing a stable platform for precise observations. The microscope offers a range of optical configurations, including brightfield, phase contrast, and differential interference contrast (DIC) modes, allowing users to adapt the instrument to their specific needs.

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Lab products found in correlation

Discovery v8, by Zeiss (2 mentions) Axiocam, by Zeiss (2 mentions) Eos 600d, by Canon (2 mentions) Em uc6, by Leica (1 mentions) Axiocam 208, by Zeiss (1 mentions) Polyclonal antibodies, by Santa Cruz Biotechnology (1 mentions) Coolpix p520, by Nikon (1 mentions)

Close spelling variants of this product

ECLIPSE 80i compound microscope

6 protocols using eclipse 80i

1

Anthoxanthum odoratum Fungal Specimen Isolation

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Specimens were collected from Anthoxanthumodoratum L. from Italy in 2013. They were examined and photographed using a Carl Zeiss Discovery V8 stereo-microscope fitted with Axiocam. Sections of ascomata were taken by hand under a stereo-microscope. Sections and other micro-morphological characters were photographed using a Nikon Eclipse 80i compound microscope fitted with a Canon 450D digital camera. All microscopic measurements were made with Tarosoft image framework (v. 0.9.0.7). Colony characteristics were recorded from cultures grown on Malt Extract Agar (MEA).
Single spore isolation was carried out following the method described by Chomnunti et al. (2014) (link). Germinated ascospores were aseptically transferred into fresh MEA plates and incubated at 20 °C to obtain pure cultures and later transferred to MEA slants and stored at 4 °C for further study. The holotype and paratype specimens were deposited at the Mae Fah Luang University (MFLU) fungaria and the herbarium of Kunming Institute of Botany, Chinese Academy of Sciences (HKAS), respectively. Living cultures were deposited at the Mae Fah Luang Culture Collection (MFLUCC). MycoBank (http://www.mycobank.org/) and Facesoffungi (Jayasiri et al. 2015 (link)) numbers were obtained for the new strain. The new species was established based on recommendations outlined by Jeewon and Hyde (2016) (link).
Senanayake I.C., Jeewon R., Camporesi E., Hyde K.D., Zeng Y.J., Tian S.L, & Xie N. (2018). Sulcisporasupratumida sp. nov. (Phaeosphaeriaceae, Pleosporales) on Anthoxanthumodoratum from Italy. MycoKeys.
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2

Zebrafish Eye Histological Processing

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Zebrafish were euthanized with tricaine methanesulfonate and the eyes enucleated and fixed overnight at 4°C in 2% PFA and 2.5% glutaraldehyde in 0.1M Sorenson phosphate buffer pH 7.3. Samples were post-fixed in 1% osmium tetroxide and dehydrated in a gradient ascending series of ethanol concentrations prior to embedding in Epon 812 resin overnight. 1 μm sections were prepared using a Leica EM UC6 microtome and glass knife, mounted on glass slides and stained with toluidine blue. The appearance of the lens core was used as a landmark to ensure similarity of samples in imaging and measuring. Sections were imaged with a Nikon Eclipse 80i upright microscope equipped with a Canon EOS 600D camera.
Carter S.P., Moran A.L., Matallanas D., McManus G.J., Blacque O.E, & Kennedy B.N. (2020). Genetic Deletion of Zebrafish Rab28 Causes Defective Outer Segment Shedding, but Not Retinal Degeneration. Frontiers in Cell and Developmental Biology, 8, 136.
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3

Microscopic Examination of Fungal Structures

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Microscopic mounts of fruiting structures in sterilized tap water were examined and photographed using a stereomicroscope fitted with a camera (ZEISS Axiocam 208). The micromorphological characteristics such as the structure of the conidiomatal wall, conidiogenous cells, and conidia were studied and photographed using a compound microscope (Nikon Eclipse 80i) fitted with a digital camera (Canon 450D). All microscopic measurements were made with the Tarosoft image framework (v. 0.9.0.7).
Kularathnage N.D., Senanayake I.C., Wanasinghe D.N., Doilom M., Stephenson S.L., Song J., Dong W, & Xu B. (2023). Plant-Associated Novel Didymellaceous Taxa in the South China Botanical Garden (Guangzhou, China). Journal of Fungi, 9(2), 182.
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4

Histological and Immunohistochemical Analysis of Kidney Tissue

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Kidney tissue samples were fixed, dehydrated, and set into paraffin before being sliced at 4 µm and added to slides stained with hematoxylin and eosin (H&E). The samples were examined and photographed for histopathological analysis using a Nikon Eclipse 80i microscope and a Canon SX620 H 20 megapixel digital camera (Awad et al., 2018 (link)). Immunohistochemical analysis was performed by embedding the slices in paraffin, rehydrating these, then soaking in H2O2 (2%) for 15 m, then inhibiting the peroxidase activity using PBS. A 5% bovine serum albumin was used to block nonspecific binding sites. Dilutions of 1:500 Bcl‐2‐associated X protein (Bax) Antibody (Catalog # sc‐23959), cyclooxygenase‐2 (Cox‐2) antibody (Catalog # sc‐19999), and polyclonal antibodies (Santa Cruz Biotechnology) were added to the slides and incubated at 4°C overnight. After washing the slides three times with PBS, a biotin‐conjugated secondary antibody (1:2000 dilution, cat# sc‐2040) was added. After developing the reaction with 3,3‐diaminobezidine tetrahydrochloride, the slides were counterstained using hematoxylin and the number of positively stained cells was compared to the total number of cells to determine how many of those cells were immunoreactive for Bax and Cox 2 (Nassan et al., 2018 (link)). Significance was determined using ANOVA for three different samples per group.
Nassan M.A., Soliman M.M., Aldhahrani A., Althobaiti F, & Alkhedaide A.Q. (2021). Ameliorative impacts of Glycyrrhiza glabra root extract against nephrotoxicity induced by gentamicin in mice. Food Science & Nutrition, 9(7), 3405-3413.
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5

Cordyceps Species Identification and Characterization

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Wireworm-infecting species of Cordyceps s.l. were collected from south-western China and the Russian Far East. Specimens were placed in plastic boxes and carried to the laboratory for further study. The macro-characteristics and ecology were photographed using a Nikon Coolpix P520 camera in the field. Specimens were examined and photographed using an Optec SZ660 stereo dissecting microscope and a Nikon Eclipse 80i compound microscope connected with a Canon EOS 600D camera. Microscopic measurements were made using Tarosoft (R) Image Framework software. Images were processed using Adobe Photoshop CS v. 8.0.1 (Adobe Systems Incorporated, San Jose, California, USA). Voucher specimens are deposited in the Fungarium of the Centre of Excellence in Fungal Research, Mae Fah Luang University (MFLU), Chiang Rai, Thailand and the Herbarium of Guizhou University (GACP), Guiyang, China.
Zha L.S., Kryukov V.Y., Ding J.H., Jeewon R, & Chomnunti P. (2021). Novel taxa and species diversity of Cordyceps sensu lato (Hypocreales, Ascomycota) developing on wireworms (Elateroidea and Tenebrionoidea, Coleoptera). MycoKeys, 78, 79-117.
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6

Isolation and Characterization of Monocotyledon Fungi

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Samples were collected in a survey of monocotyledon inhabiting fungi during 2018-2019 in Guangdong Province, China. The samples were brought to the laboratory in paper bags. They were examined and photographed using a Carl Zeiss Discovery V8 stereomicroscope fitted with Axiocam. The morphological characters were photographed using a Nikon Eclipse 80i compound microscope fitted with a Canon 450D digital camera. All microscopic measurements were made with Tarosoft image framework (v. 0.9.0.7). Colony characters were recorded from cultures grown on potato dextrose agar (PDA).
Single conidia isolation was carried out following the method described by Senanayake et al. (2018) . Germinated conidia were aseptically transferred into fresh PDA plates, and incubated at 16°C to obtain pure cultures. Cultures were later transferred to PDA slants and stored at 4°C for further studies. All the voucher specimens are deposited in the fungaria of Mae Fah Luang University (MFLU), and living cultures are deposited at the Culture Collection of Kunming Institute of Botany (KUMCC).
Senanayake I.C. (2020). New geographical records of Neopestalotiopsis and Pestalotiopsis species in Guangdong Province, China. Asian Journal of Mycology, 3(1), 512-533.
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