Phospho-EGFR (Y1068) (#3777), EGFR (#2646), phospho-AKT (S473) (#4058), AKT (#4685), phospho-ERK1/2 (T202/Y204) (#4376), ERK1/2 (#4695), and vinculin (#13901) primary antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). vinculin was used as loading control. HRP-conjugated anti-rabbit (#111-035-003) and mouse (#115-035-003) secondary antibodies were obtained from Jackson ImmunoResearch (West Grove, PA, US). Cetuximab (A2000) was purchased from Selleckchem (Houston, TX, USA). The recombinant human AREG (262-AR-100) was purchased from R&D Systems.
Cetuximab a2000
Manufactured by Selleck Chemicals
Sourced in United States
Cetuximab (A2000) is a laboratory product provided by Selleck Chemicals. It is an epidermal growth factor receptor (EGFR) inhibitor.
Automatically generated - may contain errors
Lab products found in correlation
Erk1 2, by Cell Signaling Technology (1 mentions)
Phospho erk1 2t202 y204, by Cell Signaling Technology (1 mentions)
Vinculin, by Cell Signaling Technology (1 mentions)
Phospho akt s473, by Cell Signaling Technology (1 mentions)
Phospho egfr y1068, by Cell Signaling Technology (1 mentions)
Crizotinib s1068, by Selleck Chemicals (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
3 protocols using cetuximab a2000
1
EGFR Signaling Pathway Evaluation
2
Selleck Chemicals Protocol for Drug Evaluation
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For this study, cetuximab (A2000), crizotinib (S1068), and miltefosine (S3056) were all purchased from Selleck Chemicals Llc (Houston, Texas, USA). The concentration of drugs that were used for treatment is described in Results. The drug library was designed and completed by Prof. Xingguo Zhang.
3
Tumor Microenvironment Molecular Profiling
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RPMI1640 (12633012) and FBS (10099141) were purchased from Thermo. Cetuximab (A2000) was purchased from Selleck. Human SDF-1α (ab100637), E-Cadherin (ab233611), CXCL9 (ab219047), CXCL10 (ab83700), MMP2 (ab100606) and MMP9 (ab100610) enzyme-linked immunosorbent assay (ELISA) kits were purchase from Abcam. EGFR (ab52894, 175), Ras (ab52939, 21), Raf (ab200653, 68), MEK1 (ab32091, 45), p-MEK1 (ab96379, 45), p-STAT3 (ab 76315, 87), STAT3 (ab 119352, 87), SDF-1 (ab155090, 11), CXCR4 (ab124824, 39), VEGF-α (ab53465, 27), CXCL9 (ab193851), CXCL10 (ab8098), vimentin (ab8978, 57), Fibronectin 1 (ab2413, 285).
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