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Douset elisa

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The DouSet ELISA is a laboratory instrument designed for performing enzyme-linked immunosorbent assays (ELISA). It is a versatile tool used for the quantitative detection and analysis of various analytes, such as proteins, peptides, and small molecules, in biological samples.

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Lab products found in correlation

Elisa max deluxe kit, by BioLegend (2 mentions) Mouse albumin elisa quantification kit, by Fortis Life Sciences (1 mentions)

Close spelling variants of this product

DouSet ELISA MMP-9 Kit Douset ELISA system ELISAs

4 protocols using douset elisa

1

Cytokine and ICAM Profiling in MDM

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The levels of the following cytokines were evaluated in MDM supernatants using commercially available ELISA sets: IL-8, IL-6, IL-1β, TNFα, IL-10 (ELISA MAX Deluxe kits, BioLegend) and sICAM (DouSet ELISA, R&D Systems). MIF plasma levels were evaluated using an in-house ELISA constructed with an anti-human MIF antibody pair and an MIF standard obtained from BioLegend.
Trifone C., Salido J., Ruiz M.J., Leng L., Quiroga M.F., Salomón H., Bucala R., Ghiglione Y, & Turk G. (2018). Interaction Between Macrophage Migration Inhibitory Factor and CD74 in Human Immunodeficiency Virus Type I Infected Primary Monocyte-Derived Macrophages Triggers the Production of Proinflammatory Mediators and Enhances Infection of Unactivated CD4+ T Cells. Frontiers in Immunology, 9, 1494.
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2

Cytokine Profiling in MDM Supernatants

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The levels of the following cytokines were evaluated in MDM supernatants using commercially available kits: IL-8, IL-6, IL-1β, TNFα, IL-10, IL-17A (ELISA MAX Deluxe kits, BioLegend), sICAM (DouSet ELISA, R&D Systems), IL-4, IL-2 and IFNγ (Th1/Th2 CBA kit, BD). MIF plasma levels were evaluated using an in-house ELISA constructed with an anti-human MIF antibody pair and an MIF standard obtained from BioLegend.
Trifone C., Baquero L., Czernikier A., Benencio P., Leng L., Laufer N., Quiroga M.F., Bucala R., Ghiglione Y, & Turk G. (2022). Macrophage Migration Inhibitory Factor (MIF) Promotes Increased Proportions of the Highly Permissive Th17-like Cell Profile during HIV Infection. Viruses, 14(10), 2218.
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3

Quantifying Lung Inflammation Biomarkers

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The concentration of albumin was determined via mouse albumin ELISA quantification kit (Bethyl Laboratories, Montgomery, TX). The amount of cytokines and chemokines in the BAL, lung homogenates and lung macrophages was determined via DouSet ELISA (R&D systems, Minneapolis, MN). Specifically, the amount of TGF-β production was measured in acid activated supernatants to measure total active TGF-β.
Ballinger M.N., Newstead M.W., Zeng X., Bhan U., Mo X.M., Kunkel S.L., Moore B.B., Flavell R., Christman J.W, & Standiford T.J. (2015). IRAK-M promotes alternative macrophage activation and fibroproliferation in bleomycin-induced lung injury. Journal of immunology (Baltimore, Md. : 1950), 194(4), 1894-1904.
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4

Biomarkers of Ischemic Conditioning in Acute Stroke

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Immediately before treatment and on day 4 (±1), blood samples were collected for: (1) surrogate markers of brain injury, which might be attenuated if RIC improves ischemic reperfusion injury (plasma S100ß protein, matrix metalloproteinase [MMP‐9], neuron‐specific enolase [NSE], by multiplex technology, Merck Millipore Ltd); S‐100ß, NSE, and MMP‐9 also act as surrogate markers of infarct volume and prognosis in acute ischemic stroke19, 20; and (2) HSP27 (DouSet ELISA, R&D Systems), which is a biomarker implicated in the mechanisms of ischemic conditioning10 and is neuroprotective in experimental stroke.13 Assays and analysis of data were performed blinded to treatment allocation.
England T.J., Hedstrom A., O'Sullivan S.E., Woodhouse L., Jackson B., Sprigg N, & Bath P.M. (2019). Remote Ischemic Conditioning After Stroke Trial 2: A Phase IIb Randomized Controlled Trial in Hyperacute Stroke. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 8(23), e013572.
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