Log In Sign up
The largest database of trusted experimental protocols

αcd28 clone 37

Manufactured by BioXCell
Visit Supplier

αCD28 (clone 37.51) is a monoclonal antibody that binds to the CD28 receptor on T cells. It is commonly used in cell culture and research applications. The core function of this product is to activate and stimulate T cell response.

Automatically generated - may contain errors

Lab products found in correlation

Rmil 12, by Thermo Fisher Scientific (2 mentions) Rmil 6, by Thermo Fisher Scientific (2 mentions) αifn γ clone xmg1, by BioXCell (2 mentions) αil 4 clone 11b11, by BioXCell (2 mentions) αcd3 clone 17a2, by BioXCell (2 mentions) αtgf β clone 1d11, by BioXCell (2 mentions) αil 12 clone r1 5d9, by BioXCell (2 mentions) Cd4 t cell isolation kit, by Miltenyi Biotec (2 mentions)

2 protocols using αcd28 clone 37

1

Naïve SMARTA Cell Polarization Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols
Naïve SMARTA cells were negatively isolated by using CD4+ T cell isolation kit (Miltenyi or StemCell). 2 × 106 SMARTA cells were seeded on 24 well plates coated with 8 µg/mL αCD3 (clone 17A2, BioXcell) and αCD28 (clone 37.51, BioXcell). For TH1polarization, Smarta cells were given with 20 µg/mL of αIL-4 (clone 11B11, BioXcell) and αTGF-β (clone 1D11, BioXcell) and 20 ng/mL of rmIL-12 (Peprotech). For IL-6 condition, 10 µg/mL of αIFN-γ (clone XMG1.2, BioXcell) and αIL-12 (clone R1-5D9, BioXcell) and 20 ng/mL of rmIL-6 (Peprotech) were added in culture media.
Choi Y.S., Gullicksrud J.A., Xing S., Zeng Z., Shan Q., Li F., Love P.E., Peng W., Xue H.H, & Crotty S. (2015). LEF-1 and TCF-1 orchestrate T follicular helper cell differentiation by regulating differentiation circuits upstream of Bcl6. Nature immunology, 16(9), 980-990.
+ Open protocol
+ Expand
2

Naïve SMARTA Cell Polarization Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols
Naïve SMARTA cells were negatively isolated by using CD4+ T cell isolation kit (Miltenyi or StemCell). 2 × 106 SMARTA cells were seeded on 24 well plates coated with 8 µg/mL αCD3 (clone 17A2, BioXcell) and αCD28 (clone 37.51, BioXcell). For TH1polarization, Smarta cells were given with 20 µg/mL of αIL-4 (clone 11B11, BioXcell) and αTGF-β (clone 1D11, BioXcell) and 20 ng/mL of rmIL-12 (Peprotech). For IL-6 condition, 10 µg/mL of αIFN-γ (clone XMG1.2, BioXcell) and αIL-12 (clone R1-5D9, BioXcell) and 20 ng/mL of rmIL-6 (Peprotech) were added in culture media.
Choi Y.S., Gullicksrud J.A., Xing S., Zeng Z., Shan Q., Li F., Love P.E., Peng W., Xue H.H, & Crotty S. (2015). LEF-1 and TCF-1 orchestrate T follicular helper cell differentiation by regulating differentiation circuits upstream of Bcl6. Nature immunology, 16(9), 980-990.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!