Iscove s modified dmem

Manufactured by Corning

Iscove's modified DMEM is a cell culture medium formulated to support the growth of a variety of cell types, including hematopoietic cells. It provides a balanced salt solution, amino acids, vitamins, and other components essential for cell proliferation and maintenance.

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Lab products found in correlation

Ionomycin, by Merck Group (2 mentions) Phorbol myristate acetate (pma), by Merck Group (2 mentions) Brefeldin a, by BD (2 mentions) Erythropoietin, by Thermo Fisher Scientific (1 mentions) Erythropoietin, by Amgen (1 mentions) Stem cell factor (scf), by Thermo Fisher Scientific (1 mentions) Cc100, by STEMCELL (1 mentions) Ab serum, by Atlanta Biologicals (1 mentions) Stemspan, by STEMCELL (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Insulin, by Merck Group (1 mentions) Dexamethasone (dex), by Merck Group (1 mentions)

Spelling variants of this product

Iscove’s DMEM (5 citations) Iscove’s (2 citations)

3 protocols using iscove s modified dmem

CD34+ Cell Expansion and Differentiation

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CD34⁺ expansion media: serum-free StemSpan supplemented with 10 mL/mL CC-100 (both Stemcell Technologies), 2 U/mL erythropoietin (Amgen), 10⁻⁶ M dexamethasone (Sigma) and 100 U/ml penicillin/streptomycin (GIBCO, ThermoFisher Scientific). Differentiation media: Iscove’s Modified DMEM (Cellgro) with 3% AB serum (Atlanta Biologicals), 2% human plasma (Stemcell Tech), 10 mg/mL insulin (Sigma), 3 U/mL heparin, 200 ug/mL transferrin (Athens Research & Technology), 10 ng/mL stem cell factor (Peprotech), 3 U/mL erythropoietin. Freeze media: 50% characterized fetal bovine serum (Hyclone), 10% dimethyl sulfoxide (Sigma), and 40% Iscove’s Modified DMEM. Thaw media: Iscove’s Modified DMEM supplemented with 5% characterized fetal bovine serum.

Dong A., Ghiaccio V., Motta I., Guo S., Peralta R., Freier S.M., Watt A., Damle S., Ikawa Y., Jarocha D., Chappell M., Stephanou C., Delbini P., Chen C., Christou S., Kleanthous M., Smith-Whitley K., Manwani D., Casu C., Abdulmalik O., Cappellini M.D., Rivella S, & Breda L. (2020). 2'-O-methoxyethyl splice-switching oligos correct splicing from IVS2-745 β-thalassemia patient cells restoring hemoglobin A production and chain rebalance. Haematologica, 106(5), 1433-1442.

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T Cell Differentiation Assay

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Naïve CD4⁺ T cells were purified from C57BL/6, RORγt^−/−, or RORγt^K31R/K31R mice by negative selection (Miltenyi Biotec). Suspensions of 4 × 10⁵ cells/ml Iscove’s modified DMEM (Cellgro) containing 2 mM L-glutamine, 50 mM 2-ME, 100 U/ml penicillin, 100 mg/ml streptomycin, and 10% FBS were cultured in 24-well plates pre-coated with 0.2 mg/ml goat anti-hamster antibody for three days. The medium was supplemented with 0.25 µg/ml hamster anti-CD3, 1 µg/ml hamster anti-CD28, and polarizing cytokines: 2 ng/ml TGF-β, 20 ng/ml IL-6, 5 µg/ml anti-IL-4, and 5 µg/ml anti-IFNγ for T_H17 differentiation; 20 µg/ml IL-12 and 5 µg/ml anti-IL-4 for Th1 differentiation; 10 ng/ml IL-4 and 10 µg/ml anti-IFNγ for T_H2 differentiation; or 5 ng/ml TGF-β for Treg differentiation. For analysis, cells obtained from in vitro cultures were incubated for 4–5 h with 50 ng/ml PMA (Sigma-Aldrich), 750 ng/ml ionomycin (Sigma-Aldrich), and 10 µg/ml brefeldin A (BD Biosciences) in a tissue culture incubator at 37 °C, followed by intracellular cytokine staining.

He Z., Zhang J., Huang Z., Du Q., Li N., Zhang Q., Chen Y, & Sun Z. (2018). Sumoylation of RORγt regulates TH17 differentiation and thymocyte development. Nature Communications, 9, 4870.

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Differentiation of Murine CD4+ T-cell Subsets

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Naïve CD4⁺ T cells were purified from C57BL/6, Rorγt^−/−, Src3^+/−, or Src3^−/− mice by negative selection (Miltenyi Biotec). Suspensions of 4×10⁵ cells/ml Iscove’s modified DMEM (Cellgro) containing 2 mM L-glutamine, 50 mM 2-ME, 100 U/ml penicillin, 100 mg/ml streptomycin, and 10% FBS were cultured in 24-well plates pre-coated with 0.2 mg/ml goat anti-hamster antibody for three days. The medium was supplemented with 0.25 μg/ml hamster anti-CD3, 1 μg/ml hamster anti-CD28, and polarizing cytokines: 2 ng/ml TGFβ, 20 ng/ml IL-6, 5 μg/ml anti-IL-4, and 5 μg/ml anti-IFNγ for Th17 differentiation; 20 μg/ml IL-12 and 5 μg/ml anti-IL-4 for Th1 differentiation; 10 ng/ml IL-4 and 10 μg/ml anti- IFNγ for Th2 differentiation; or 5 ng/ml TGFβ for Treg differentiation. For analysis, cells obtained from in vitro cultures were incubated for 4–5 h with 50 ng/ml PMA (Sigma-Aldrich), 750 ng/ml ionomycin (Sigma-Aldrich), and 10 μg/ml brefeldin A (BD Biosciences) in an incubator at 37°C, followed by intracellular cytokine staining.

He Z., Zhang J., Du Q., Xu J., Gwack Y, & Sun Z. (2018). SRC3 is a co-factor for RORγt in Th17 differentiation but not thymocyte development. Journal of immunology (Baltimore, Md. : 1950), 202(3), 760-769.

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