The FISH protocol was described previously (21 ) and used a bacterial artificial chromosome (BAC) spanning the CFTR locus (22 (link)). Probes were biotin-labeled by nick translation and signals were detected using an anti-biotin antibody conjugated to fluorescein (Jackson Laboratories). DAPI was used as the nuclear stain.
Anti biotin antibody
Manufactured by Jackson ImmunoResearch
Anti-biotin antibody is a laboratory reagent used for the detection and identification of biotin-labeled molecules. It binds specifically to biotin, a small molecule label commonly used in various biological assays and techniques.
Automatically generated - may contain errors
Lab products found in correlation
Anti beta 3 tubulin, by Merck Group (1 mentions)
Hoechst 33342, by Thermo Fisher Scientific (1 mentions)
Streptavidin cy3, by Thermo Fisher Scientific (1 mentions)
Bdnf b 250, by Alomone (1 mentions)
Anti bdnf n 20, by Santa Cruz Biotechnology (1 mentions)
Anti flag, by Merck Group (1 mentions)
Iaa i2886, by Merck Group (1 mentions)
Anti his antibody, by Jackson ImmunoResearch (1 mentions)
3 protocols using anti biotin antibody
1
Fluorescent In-Situ Hybridization for CFTR
2
BDNF Signaling Pathway Analysis
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For phospho-Trk and phospho-Erk activation, CGN and PC12 cells were incubated in serum-free DME supplemented with 0.1% bovine serum albumin (BSA) and 2mM L-glutamine and 100μg/ml penicillin and 100μg/ml streptomycin (DMEB) for 1h prior to treatment with BDNF, proBDNF and HBpF-proBDNF. The anti-biotin antibody was from Jackson Immunoresearch, anti-Flag and anti-beta III-tubulin were from Sigma, anti-BDNF (N-20) was from Santa-Cruz Biotechnology, Inc. and anti phospho-TrkA (Tyr490) was from Cell Signaling Technology. Antibodies against p75NTR, total TrkA and total TrkB have been previously described [20 (link)–23 (link)]. Anti-Sortilin and anti-SorCS2 were gifts from Dr. Barbara Hempstead, Rhodamine-tagged phalloidin was from Life technologies and Hoechst 33342 was obtained from Molecular Probes. Horseradish-peroxidase-conjugated and fluorescent dye-conjugated secondary antibodies were purchased from Jackson ImmunoResearch (West Grove, PA). BDNF (B250) and proBDNF (B240) was purchased from Alomone labs (Jerusalem, Israel). Cy3-Streptavidin was purchased from Invitrogen and GM6001 was purchased from Calbiochem.
3
Arabidopsis and Rice Growth Conditions
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Growth conditions for Arabidopsis and rice Nipponbare (Oryza sativa L. ssp. japonica) were described previously (Yang et al., 2012; Qi et al., 2015a Qi et al., , 2015b;; Huang et al., 2017) . Coronatine (C8115), jasmonic acid (J2500), MeJA (S4400), and IAA (I2886) were purchased from Sigma. Anti-Biotin antibody and anti-His antibody were purchased from Jackson ImmunoResearch and Sigma, respectively.
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