Nci h1703 h1703 cells

Manufactured by American Type Culture Collection

Sourced in United States

NCI-H1703 [H1703] cells are a lung cancer cell line derived from a human male. The cells are adherent in nature and can be used for various research applications.

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Lab products found in correlation

Mycoscope pcr mycoplasma detection kit, by Genlantis (2 mentions) Rpmi 1640 medium, by American Type Culture Collection (2 mentions) F 12k medium, by American Type Culture Collection (2 mentions) Nci h1703, by American Type Culture Collection (1 mentions) Penicillin streptomycin solution, by American Type Culture Collection (1 mentions) A549 cell, by American Type Culture Collection (1 mentions)

2 protocols using nci h1703 h1703 cells

Characterization of Lung Cancer Cell Lines

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Human lung adenocarcinoma A549 cells (#CCL-185, passage number 1-12) and human lung squamous cell carcinoma NCI-H1703 [H1703] cells (#CRL-5889, passage number 1-12) were purchased from ATCC (Manassas, VA, USA). A549 clonal cell lines, 1-40 and 2-11, were obtained from Bialk et al [6 (link)]. Cells were thawed, according to the manufacturer’s protocol. A549 cells were grown in F-12K medium (ATCC) supplemented with 10% fetal bovine serum (FBS) (ATCC). NCI-H1703 cells were grown in RPMI 1640 medium (ATCC) supplemented with 10% fetal bovine serum (FBS) (ATCC). Both cell lines were grown at 37 °C in 5% CO₂. Cell lines were tested for Mycoplasma upon thawing and before use in experiments using the MycoScope PCR Mycoplasma detection kit (Genlantis, Cat. MY01100).

Banas K., Modarai S., Rivera-Torres N., Yoo B.C., Bialk P.A., Barrett C., Batish M, & Kmiec E.B. (2022). Exon skipping induced by CRISPR-directed gene editing regulates the response to chemotherapy in non-small cell lung carcinoma cells. Gene Therapy, 29(6), 357-367.

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Characterization of Lung Cancer Cell Lines

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Human lung adenocarcinoma A549 cells (#CCL-185, passage number 1–12) and Human lung squamous cell carcinoma NCI-H1703 [H1703] cells (#CRL-5889, passage number 1–12) were purchased from ATCC (Manassas, VA, USA). Cells were thawed, according to the manufacturer’s protocol. A549 cells were grown in F-12K medium (ATCC) supplemented with 10% fetal bovine serum (FBS) (ATCC) and 1% Penicillin-Streptomycin Solution (ATCC). NCI-H1703 cells were grown in RPMI 1640 medium (ATCC) supplemented with 10% fetal bovine serum (FBS) (ATCC) and grown at 37°C in 5% CO₂. Both cell lines were tested for Mycoplasma upon thawing and before use in experiments using the MycoScope PCR Mycoplasma detection kit (Genlantis, Cat. MY01100). The A549 cell line was previously characterized by ATCC using Short Tandem Repeat (STR) profiling, cellular morphology and karyotyping. The H1703 cell line was previously characterized by ATCC using STR profiling and cellular morphology. No further authentication was performed.

Banas K., Rivera-Torres N., Bialk P., Yoo B.C, & Kmiec E.B. (2020). Kinetics of nuclear uptake and site-specific DNA cleavage during CRISPR-directed gene editing in solid tumor cells. Molecular cancer research : MCR, 18(6), 891-902.

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