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Ex52735

Manufactured by Merck Group
Sourced in United States

The Ex52735 is a laboratory equipment product manufactured by Merck Group. It is designed to perform a core function in a laboratory setting. However, a detailed and unbiased description of its specific features and capabilities is not available at this time.

Automatically generated - may contain errors

2 protocols using ex52735

1

Molecular Mechanisms of GN Regulation

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GN (≥98% purity) was obtained from ChemFaces (Wuhan, China). Antibodies against SREBP-1c (H-160; sc-8984), PPARα (H-2; sc-398394), PGC-1α (H-300; sc-13067), IκBα (H-4; sc-164), NF-κB p65 (F-6; sc-8008p65), SIRT1 (H-300; sc-15404), AMPK α1/2 (D-6; sc-74461), and β-actin (C4; sc-47778) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Specific primary antibodies against p-ACC (Ser79; #3661s) and ACC (C83B10; #3676s) were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibodies against CYP2E1 (ab28146) and p-AMPK α1 (Thr172; PA5-17831) were purchased from Abcam (Cambridge, MA, USA) and Invitrogen (Rockford, IL, USA), respectively. Ethanol and Ex52735 were obtained from Sigma (St. Louis, MO, USA).
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2

Cryptotanshinone Signaling Pathway Modulation

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Cryptotanshinone (≥98% purity) was obtained from ChemFaces (Wuhan, China). Antibodies against SREBP-1c (H-160; sc-8984), PPARα (H-2; sc-398394), IkBα (H-4; sc-164), NFκB p65 (F-6; sc-8008p65), AMPK α1/2 (D-6; sc-74461), SIRT1 (H-300; sc-15404), and β-actin (C4; sc-47778) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Specific primary antibodies against p-ACC (Ser79; #3661s) and ACC (C83B10; #3676s) were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibodies against CYP2E1 (ab28146) and pAMPK α1 (Thr172; PA5-17831) were purchased from Abcam (Cambridge, MA, USA) and Invitrogen (Rockford, IL, USA), respectively. The Nrf2 antibody (NBP1-32822) was obtained from Novus Biologicals (Centennial, CO, USA). Ethanol, Ex52735, and compound C were obtained from Sigma (St. Louis, MO, USA).
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