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Aura software

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Aura software is a core application for Spectral Instruments Imaging's lab equipment. It provides a user interface to control and manage the operation of the hardware.

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Lab products found in correlation

Living image software 4, by PerkinElmer (3 mentions) Ivis spectrum imaging system, by PerkinElmer (3 mentions) Ivis spectrum in vivo imaging system, by PerkinElmer (2 mentions) Rediject d luciferin ultra bioluminescent substrate, by PerkinElmer (2 mentions) Lago x optical imaging system, by Spectral Instruments Imaging (2 mentions) Living image software, by PerkinElmer (2 mentions) Ami led illumination based imaging system, by Spectral Instruments Imaging (2 mentions) Myeloperoxidase inhibitor screening assay kit, by Cayman Chemical (2 mentions) Kino imaging system, by Spectral Instruments Imaging (2 mentions) Luminol, by Merck Group (2 mentions) C57bl 6j mice, by Jackson ImmunoResearch (2 mentions) Ami htx, by Spectral Instruments Imaging (2 mentions) D luciferin, by GoldBio (2 mentions) Htx in vivo imaging system, by Spectral Instruments Imaging (2 mentions) Pierce bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Ami imager, by Spectral Instruments Imaging (1 mentions) Celltrace violet, by Thermo Fisher Scientific (1 mentions) Lago x imaging system, by Spectral Instruments Imaging (1 mentions) Luciferase 1000 assay system, by Promega (1 mentions) Bullet blender, by Next Advance (1 mentions)

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Aura imaging software (25 citations) Aura Image software (2 citations)

25 protocols using aura software

1

In Vivo Bioluminescent Imaging of Mice

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Prior to imaging, the mice were i.p. injected with 100 μL of 30 mg/ml RediJect D-Luciferin Ultra bioluminescent substrate (PerkinElmer). Mice were then anesthetized with isoflurane and imaged within 20 minutes for bioluminescence using the in vivo imager. All the bioluminescence data, except for those shown in Supplementary Figures 7d and e, were collected using the IVIS Spectrum In Vivo Imaging System (PerkinElmer), and bioluminescent photon outputs were quantified using the Living Image Software (PerkinElmer). The bioluminescence data shown in Supplementary Figures 7d and e were collected using the Lago X optical imaging system (Spectral Instruments Imaging) and analyzed using Aura software (Spectral Instruments Imaging).
Zhong G., Wang H., He W., Li Y., Mou H., Tickner Z.J., Tran M.H., Ou T., Yin Y., Diao H, & Farzan M. (2019). A reversible RNA on-switch that controls gene expression of AAV-delivered therapeutics in vivo. Nature biotechnology, 38(2), 169-175.
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2

In Vivo Bioluminescence Imaging

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Mice were anesthetized using 2% isoflurane and injected intraperitoneally with d-luciferin (10 mg/kg). Bioluminescence imaging (BLI) was performed using the IVIS Spectrum imaging system (Perkin Elmer), and data were analyzed with Living Image Software 4.1 (Perkin Elmer) or using an Ami LED-illumination–based imaging system (Spectral Instruments Imaging, Tucson, AZ) and analyzed with Aura Software (Spectral Instruments Imaging).
Xiao Z., Alam I.S., Simonetta F., Chen W., Scheller L., Murty S., Lohmeyer J.K., Ramos T.L., James M.L., Negrin R.S, & Gambhir S.S. (2022). ICOS immunoPET enables visualization of activated T cells and early diagnosis of murine acute gastrointestinal GvHD. Blood Advances, 6(16), 4782-4792.
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3

Preclinical Evaluation of CRISPR-engineered NK Cells

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The next day after CRISPR electroporation, 1 × 106 NK cells were washed with PBS and injected into NSG mice i.v.; the cells were then supported with 1 μg rhIL-15 i.p. 3 times per week. For K562 tumor challenge experiments, approximately 1.5 × 106 luciferase-expressing K562 cells were injected i.v. 4 days after NK cell injection, and 100 ng/mouse rhIL-15 was used to support NK cells for the duration of the experiment after tumor injection. Bioluminescent imaging (BLI) was performed twice a week on an AMI imager (Spectral Instruments Imaging) 10 minutes after i.p. injection of 150 mg/kg d-luciferin. Quantification of BLI signals was performed using Aura software (Spectral Instruments Imaging).
For proliferation assessment, NK cells were washed with PBS and incubated with 1:2,000 CellTrace Violet (Invitrogen) following the manufacturer’s protocol for labeling before injection into the mice. Dye dilution was tracked at time of mouse harvesting by flow cytometry.
For experiments assessing persistence, proliferation, and ex vivo functionality, NK cells were maintained with 1 μg/mouse rhIL-15 i.p. 3 times per week for the entire course of the study.
Wong P., Foltz J.A., Chang L., Neal C.C., Yao T., Cubitt C.C., Tran J., Kersting-Schadek S., Palakurty S., Jaeger N., Russler-Germain D.A., Marin N.D., Gang M., Wagner J.A., Zhou A.Y., Jacobs M.T., Foster M., Schappe T., Marsala L., McClain E., Pence P., Becker-Hapak M., Fisk B., Petti A.A., Griffith O.L., Griffith M., Berrien-Elliott M.M, & Fehniger T.A. (2023). T-BET and EOMES sustain mature human NK cell identity and antitumor function. The Journal of Clinical Investigation, 133(13), e162530.
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4

Bioluminescence Imaging of S. aureus Infection

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BLI was performed by using a Lago X imaging system (Spectral Instruments Imaging). Mice infected with luciferase-expressing XEN36 S. aureus were anesthetized with 2.5% isoflurane, and bioluminescent signals were quantified by using Aura software (Spectral Instruments Imaging). The acquisition parameters were adjusted as follows; exposure time 30 seconds, binning low (2 (link)), f/stop 1.2, and FOV 25. The luminescence data was analyzed using Aura software.
Hajfathalian M., de Vries C.R., Hsu J.C., Amirshaghaghi A., Dong Y.C., Ren Z., Liu Y., Huang Y., Li Y., Knight S.A., Jonnalagadda P., Zlitni A., Grice E.A., Bollyky P.L., Koo H, & Cormode D.P. (2023). Theranostic gold-in-gold cage nanoparticles enable photothermal ablation and photoacoustic imaging in biofilm-associated infection models. The Journal of Clinical Investigation, 133(21), e168485.
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5

In Vivo Xenograft Tumor Imaging

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Mouse experiments were conducted and approved by the Administrative Panel on Laboratory Animal Care of Stanford University. Nude mice (NU/J) were acquired from The Jackson Laboratory (#002019). The animals were kept in the animal facility with a normal night/day cycle and on autoclaved chow ad libitum. HeLa cells were grown in 225-cm2 flasks at ∼90% confluence and >90% viability before transplantation. On the day of transplantation, cells were harvested and resuspended in PBS. Cells (5 × 106) were injected subcutaneously in the animal flank in 200 μL of sterile PBS. AAV injections were performed subcutaneously using 5 × 109 vector genomes/mouse. In vivo luciferase imaging was performed using the Lago optimal imaging system (Spectral Instruments Imaging). Luciferase images were analyzed using Aura Software (Spectral Instruments Imaging). Explanted liver and HeLa tumor were freshly homogenized in PBS using a Bullet Blender (Next Advance). The luciferase assay was performed using the Promega Luciferase 1000 Assay System (Promega, #E4550) following the manufacturer’s instructions. Protein concentration in tissue samples was measured with a Pierce BCA protein assay kit (Thermo Fisher, #23227) following the manufacturer’s instructions.
Puzzo F., Zhang C., Powell Gray B., Zhang F., Sullenger B.A, & Kay M.A. (2023). Aptamer-programmable adeno-associated viral vectors as a novel platform for cell-specific gene transfer. Molecular Therapy. Nucleic Acids, 31, 383-397.
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6

In vivo Bioluminescence Imaging Protocol

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In vivo bioluminescence imaging (BLI) was performed as previously described (31 (link)). Briefly, mice were injected with luciferin (10 mg/kg; IP), anesthetized, and imaged. Imaging was conducted using an IVIS Spectrum charge coupled device imaging system (Caliper-Xenogen, Alameda, CA) and data analysis with Living Image Software (Caliper Life Sciences, Hopkinton, MA) or using an Ami LED-illumination based imaging system (Spectral Instruments Imaging, Tucson, AZ) and data analysis with Aura Software (Spectral Instruments Imaging).
Mavers M., Simonetta F., Nishikii H., Ribado J.V., Maas-Bauer K., Alvarez M., Hirai T., Turkoz M., Baker J, & Negrin R.S. (2019). Activation of the DR3-TL1A Axis in Donor Mice Leads to Regulatory T Cell Expansion and Activation With Reduction in Graft-Versus-Host Disease. Frontiers in Immunology, 10, 1624.
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7

Recombinant Scl1 Protein MPO Inhibition

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Recombinant Scl1 proteins were examined for MPO activity using the Myeloperoxidase Inhibitor Screening Assay Kit (700170, Cayman Chemical, Ann Arbor, MI). The proteins were reconstituted in PBS and used in the assay according to the manufacturer’s protocol.
MPO activity was also assessed by luminol bioluminescence imaging of isolated bone marrow neutrophils as described previously (35 (link), 36 (link)). Briefly, 1x105 isolated neutrophils were added to a 96-well black walled plate and stained with 100mM luminol (Sigma-Aldrich, MO, USA) followed by stimulation with 500nM phorbol 12-myristate 13-acetate, 99+% (Thermo Scientific Chemicals). Immediately following stimulation, cells were imaged for 60 minutes using the Kino imaging system (Spectral Instruments Imaging, AZ, USA) at 37°C under 5% CO2 flow for 12 total acquisitions. Data was analyzed by ROI measurements using Aura software (Spectral Instruments Imaging, AZ, USA) imported into Excel (Microsoft Corp., WA, USA). Data was represented as total flux (photons/second) by averaging triplicate wells and calculating the area under the curve from images taken at 5-60 min.
Henderson E.A., Ivey A., Choi S.J., Santiago S., McNitt D., Liu T.W., Lukomski S, & Boone B.A. (2024). Group A streptococcal collagen-like protein 1 restricts tumor growth in murine pancreatic adenocarcinoma and inhibits cancer-promoting neutrophil extracellular traps. Frontiers in Immunology, 15, 1363962.
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8

Heterotopic Heart Transplantation in Newborn Mice

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Heterotopic heart transplantation was performed as described previously (1 (link)). Briefly, newborn donors and recipients were anesthetized with isoflurane. Obtained newborn hearts were placed into ear pinna through the tunnel made from small incision on occiput skin by using 14-gauge 1” to 1/4” Jelco IV Catheter (Smiths Medical). Heart graft viability was assessed with daily visual observation. In case of luc+-donor, BLI was performed every 2 days from d0. D-luciferin (Biosynth) was injected i.p. 10 minutes prior to image acquisition with Ami Imager. Firefly luciferase images were analyzed with Aura software (Spectral Instruments Imaging).
Hirai T., Mayer A.T., Nobashi T.W., Lin P.Y., Xiao Z., Udagawa T., Seo K., Simonetta F., Baker J., Cheng A.G., Negrin R.S, & Gambhir S.S. (2021). Imaging alloreactive T cells provides early warning of organ transplant rejection. JCI Insight, 6(13), e145360.
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9

In Vivo Bioluminescence Imaging Protocols

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In vivo bioluminescence imaging (BLI) was performed as previously described (27 (link)), using an IVIS Spectrum imaging system (Perkin Elmer) and Living Image Software 4.1 (Perkin Elmer) or using an Ami LED-illumination based imaging system (Spectral Instruments Imaging) with Aura Software (Spectral Instruments Imaging).
Simonetta F., Lohmeyer J.K., Hirai T., Maas-Bauer K., Alvarez M., Wenokur A.S., Baker J., Aalipour A., Ji X., Haile S., Mackall C.L, & Negrin R.S. (2021). Allogeneic CAR Invariant Natural Killer T Cells Exert Potent Antitumor Effects through Host CD8 T-Cell Cross-Priming. Clinical Cancer Research, 27(21), 6054-6064.
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10

Biodistribution of Chitosan Nanoparticles

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To assess the biodistribution of CS-NP semi-quantitatively, 6–7 week old male and female C57BL/6J mice (Jackson Laboratories, Bar Harbor, ME, USA) were orally gavaged with 10 mg/kg of rhodamine loaded in 200 uL of 500 μM CS-NP R, 10 mg/kg of free R, or PBS control. Mice were euthanized after 3, 24, or 48 hours post-injection and organs (e.g., brain, heart, lungs, liver, kidneys, spleen, intestines, and bladder) were excised and imaged ex vivo on an AMI HTX in vivo imaging system (Spectral Instruments Imaging, Tuscon, AZ, USA). The fluorescence signal was quantified via Aura software (Spectral Instruments Imaging, Tuscon, AZ, USA, N ≥ 4), and background was subtracted from the PBS-treated group. The mean radiance (photons/s/cm2/sr) for each organ was quantified as a region of interest, and % of total organ fluorescence was obtained by dividing each organ by the sum of all the organ regions. Urine and blood samples were collected following organ harvest and stored at −20°C until further analysis. All animal procedures followed NIH guidelines for the care and use of laboratory animals and were approved by the University of Southern California’s Institutional Animal Care and Use Committee.
Wang J., Chin D., Poon C., Mancino V., Pham J., Li H., Ho P.Y., Hallows K.R, & Chung E.J. (2020). Oral Delivery of Metformin by Chitosan Nanoparticles for Polycystic Kidney Disease. Journal of controlled release : official journal of the Controlled Release Society, 329, 1198-1209.
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